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Apc tim3 clone f38 2e2

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APC-TIM3 (clone F38-2E2) is a fluorochrome-conjugated monoclonal antibody that recognizes the T-cell immunoglobulin and mucin domain-containing protein 3 (TIM-3) expressed on various immune cells. It is commonly used in flow cytometry applications to identify and analyze TIM-3-positive cell populations.

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Lab products found in correlation

Fitc cd34 clone 561, by BioLegend (2 mentions) Pe cd38 clone hit2, by BioLegend (2 mentions) Apc cd45 clone hi30, by BioLegend (2 mentions) Pacific blue lineage cocktail, by BioLegend (2 mentions) Pacific blue cd138 clone mi15, by BioLegend (2 mentions) Apc cy7 cd90 clone 5e10, by BioLegend (2 mentions) Bv421 cd99 clone 3b2 ta8, by BioLegend (2 mentions) Pacific blue cd123 clone 6h6, by BioLegend (2 mentions) Facsaria 3, by BD (2 mentions)

Close spelling variants of this product

Clone F38-2E2 TIM3-APC

2 protocols using apc tim3 clone f38 2e2

1

Isolation of HSC, LSC-enriched, and non-LSC-enriched Populations from AML Cells

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Human AML cells at a concentration of 1 million cells/mL were sorted on BD FACS Aria III to isolate immunophenotypically defined HSC (CD45dimSSclowLin–CD90+CD34+CD38–), LSC-enriched (CD45dimSSclow Lin–CD90–CD34+CD38–), and non-LSC-enriched (CD45dimSSclowLin–CD90–CD34+CD38+) populations. The anti-human antibodies used for FACS analysis were FITC-CD34 (clone 561, Biolegend), PE-CD38 (clone HIT2, Biolegend), APC-CD45 (clone HI30, Biolegend), Pacific Blue-Lineage cocktail (#348805, Biolegend) combined with Pacific blue-CD138 (clone MI15, Biolegend) to exclude plasma cells, APC/Cy7-CD90 (clone 5E10, Biolegend), BV421-CD99 (clone 3B2/TA8, Biolegend), Pacific Blue-CD123 (clone 6H6, Biolegend), and APC-TIM3 (clone F38-2E2, Biolegend). Briefly, cryopreserved or fresh mononuclear cells from AML patients were stained with antibody cocktail on ice for 30 min before being washed once and resuspended in sorting buffer. A high purity single cell sorting strategy was used for cell numbers less than 500 into PCR strip tubes. Bulk leukemic blasts (>20,000 cells) were sorted into 1.5 mL Eppendorf tubes. Purity of sorting achieved for all samples were >98%.
Saygin C., Hu E., Zhang P., Sher S., Lozanski A., Doong T.J., Nicolet D., Orwick S., Labanowska J., Skinner J.N., Cempre C., Kauffman T., Goettl V.M., Heerema N.A., Abruzzo L., Miller C., Lapalombella R., Behbehani G., Mims A.S., Larkin K., Grieselhuber N., Walker A., Bhatnagar B., Bloomfield C.D., Byrd J.C., Lozanski G, & Blachly J.S. (2021). Genomic analysis of cellular hierarchy in acute myeloid leukemia using ultrasensitive LC-FACSeq. Leukemia, 35(12), 3406-3420.
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2

Isolation of AML cell populations

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Human AML cells at a concentration of 1 million cells/mL were sorted on BD FACS Aria III to isolate immunophenotypically defined HSC (CD45dimSSclowLin–CD90+CD34+CD38–), LSC-enriched (CD45dimSSclow Lin–CD90–CD34+CD38–), and non-LSC-enriched (CD45dimSSclowLin–CD90–CD34+CD38+) populations. The anti-human antibodies used for FACS analysis were FITC-CD34 (clone 561, Biolegend), PE-CD38 (clone HIT2, Biolegend), APC-CD45 (clone HI30, Biolegend), Pacific Blue-Lineage cocktail (#348805, Biolegend) combined with Pacific blue-CD138 (clone MI15, Biolegend) to exclude plasma cells, APC/Cy7-CD90 (clone 5E10, Biolegend), BV421-CD99 (clone 3B2/TA8, Biolegend), Pacific Blue-CD123 (clone 6H6, Biolegend), and APC-TIM3 (clone F38–2E2, Biolegend). Briefly, cryopreserved or fresh mononuclear cells from AML patients were stained with antibody cocktail on ice for 30 minutes before being washed once and resuspended in sorting buffer. A high purity single cell sorting strategy was used for cell numbers less than 500 into PCR strip tubes. Bulk leukemic blasts (>20,000 cells) were sorted into 1.5 mL Eppendorf tubes. Purity of sorting achieved for all samples were >98%.
Saygin C., Hu E., Zhang P., Sher S., Lozanski A., Doong T.J., Nicolet D., Orwick S., Labanowska J., Skinner J.N., Cempre C., Kauffman T., Goettl V.M., Heerema N.A., Abruzzo L., Miller C., Lapalombella R., Behbehani G., Mims A.S., Larkin K., Grieselhuber N., Walker A., Bhatnagar B., Bloomfield C.D., Byrd J.C., Lozanski G, & Blachly J.S. (2021). Genomic analysis of cellular hierarchy in acute myeloid leukemia using ultrasensitive LC-FACSeq. Leukemia, 35(12), 3406-3420.
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