Anti slow clone m8421

Manufactured by Merck Group

Anti-slow (clone M8421) is a laboratory reagent used for the detection and quantification of a specific protein or analyte in biological samples. It is a monoclonal antibody that binds to and identifies the target molecule. The core function of this product is to serve as a tool for researchers and scientists in their analytical and experimental work, providing a reliable and consistent way to measure the presence or concentration of the desired target.

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Lab products found in correlation

Histofine simple stain max po m, by Nichirei Biosciences (2 mentions) Anti fast clone m4276, by Merck Group (2 mentions) Cryostat microtome, by Sakura Finetek (1 mentions) Vhx 5000, by Keyence (1 mentions) Cryostat microtome, by Leica camera (1 mentions) Image software, by Techcomp Instruments (1 mentions)

2 protocols using anti slow clone m8421

Skeletal Muscle Fiber Typing in Piglets

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Longissimus thoracis muscles obtained from 40-day-old piglets were frozen in dry-ice acetone (−78°C), and cryosections were generated using a cryostat microtome (Sakura Finetek). To analyze the distribution of skeletal muscle fiber types, the sections were immunostained with anti-slow (clone M8421, 1:400; Sigma) and anti-fast (clone M4276, 1:200; Sigma) myosin heavy-chain monoclonal antibodies, which are specific markers of type I and type II myofibers, respectively. Histofine Simple Stain MAX PO (M) (Nichirei) was used as the secondary antibody. The proportion of each fiber type in each section of the longissimus thoracis muscle was determined using a digital microscope (VHX-5000; Keyence).

Tanihara F., Takemoto T., Kitagawa E., Rao S., Do L.T., Onishi A., Yamashita Y., Kosugi C., Suzuki H., Sembon S., Suzuki S., Nakai M., Hashimoto M., Yasue A., Matsuhisa M., Noji S., Fujimura T., Fuchimoto D.I, & Otoi T. (2016). Somatic cell reprogramming-free generation of genetically modified pigs. Science Advances, 2(9), e1600803.

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Determining Skeletal Muscle Fiber Types

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Gastrocnemius and soleus muscles obtained from 14 week old mice were frozen in acetone cooled by dry ice. In order to determine skeletal muscle fiber type, the cryosections were cut using a cryostat microtome (Leica, Wetzlar, Germany) and subjected to immunohistochemistry. The sections were immunostained with anti-slow (clone M 8421, 1:600, Sigma) and anti-fast (clone M 4276, 1:300, Sigma) myosin heavy chain monoclonal antibodies, specific markers of type-I and type-II myofibers, respectively. Histofine Simplestain MAX-PO (M) (Nichirei, Tokyo, Japan) was used as the secondary antibody. The proportion of each fiber type was determined in each section of the gastrocnemius and soleus muscles using a photomicroscope photograph (Keyence) and Scion Image software.

Watanabe H., Nakano T., Saito R., Akasaka D., Saito K., Ogasawara H., Minashima T., Miyazawa K., Kanaya T., Takakura I., Inoue N., Ikeda I., Chen X., Miyake M., Kitazawa H., Shirakawa H., Sato K., Tahara K., Nagasawa Y., Rose M.T., Ohwada S., Watanabe K, & Aso H. (2016). Serotonin Improves High Fat Diet Induced Obesity in Mice. PLoS ONE, 11(1), e0147143.

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