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αcd62l

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αCD62L is a monoclonal antibody that specifically binds to the CD62L (L-selectin) cell surface antigen. CD62L is expressed on the surface of various leukocyte subsets, including T cells, B cells, and neutrophils. The αCD62L antibody can be used to detect and quantify the expression of CD62L on these cell populations.

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Lab products found in correlation

α cd8, by Thermo Fisher Scientific (3 mentions) α cd44, by Thermo Fisher Scientific (3 mentions) α cd4, by Thermo Fisher Scientific (2 mentions) Fc receptor block, by Miltenyi Biotec (1 mentions) αcd28, by BD (1 mentions) α foxp3, by Thermo Fisher Scientific (1 mentions) Facsaria, by BD (1 mentions) Golgiplug, by BD (1 mentions) αifn γ, by Thermo Fisher Scientific (1 mentions) Mouse cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions) Dulbecco s modified eagle medium dmem medium, by Thermo Fisher Scientific (1 mentions) α cd80, by Thermo Fisher Scientific (1 mentions) α cd86, by Thermo Fisher Scientific (1 mentions) α mhcii, by Thermo Fisher Scientific (1 mentions) αcd11c, by Thermo Fisher Scientific (1 mentions) Dimethyldioctadecylammonium bromide ddab, by Merck Group (1 mentions) Ovalbumin ova257 264, by Merck Group (1 mentions) α cd19, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

αCD62L-APC (MEL-14, (2 citations)

4 protocols using αcd62l

1

Multiparametric Flow Cytometry Analysis

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Relative frequency of expression of specific markers were analysed using antibodies specific for αCD3, αCD8, αCD45RA, αCD27, αCD28, αCCR7 (BD Biosciences) and αCD62L (eBiosciences, USA). Where PBMCs analysed, Fc receptor block was used (Miltenyi Biotec, Germany). To detect DMF5 TCR expression, a MART-1/HLA*0201 antigen specific pentamer was used.
Kueberuwa G., Gornall H., Alcantar-Orozco E.M., Bouvier D., Kapacee Z.A., Hawkins R.E, & Gilham D.E. (2017). CCR7+ selected gene-modified T cells maintain a central memory phenotype and display enhanced persistence in peripheral blood in vivo. Journal for Immunotherapy of Cancer, 5, 14.
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2

Detailed Workflow for Murine T Cell Analysis

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Isolated cells were stained with αCD4, αCD8, αCD3, αTCRβ, αCD44, αCD62L, αFoxP3, αIL-17 and αIFN-γ (eBioscience and Tombo). For FoxP3 and cytokine staining, cells were stained according to the manufacturer’s protocol form FoxP3 staining kit (Affimetrix/eBioscience). For cytokine staining, cells were stimulated with 50 ng/ml PMA and 1 μg/ml ionomycin in the presence of GolgiPlug (BD Bioscience) for 4 hours at 37°C and stained for surface markers followed by fixation/permeabilization and staining for cytokines. Stained cells were acquired with LSRII or LSR Fortessa cytometers; data was analyzed using FlowJo (Treestar) (BD Bioscience). For sorting of Treg cells, CD4+ T cells were enriched by using mouse CD4+ T cell isolation kit (Miltenyi) or Magnisort mouse CD4+ T cell enrichment kit (Affimetrix/eBioscience) and then cells were sorted by FACSAria (BD Bioscience).
Oh H., Grinberg-Bleyer Y., Liao W., Maloney D., Wang P., Wu Z., Wang J., Bhatt D.M., Heise N., Schmid R.M., Hayden M.S., Klein U., Rabadan R, & Ghosh S. (2017). An NF-κB transcription factor-dependent, lineage specific transcriptional program promotes regulatory T cell identity and function. Immunity, 47(3), 450-465.e5.
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3

PLGA-Based Antigen Delivery System

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Poly (D, L-lactic-co-glycolic acid) (75:25 PLGA, Resomer® RG 752H, MW 4A) was purchased from Lakeshore Biomaterials (Birmingham, AL, USA). Ovalbumin (OVA257-264) and dimethyl-dioctadecyl-ammonium bromide (DDAB) were obtained from Sigma (St, Louis, MO, USA). Methylene chloride (AR grade) and absolute ethyl alcohol (AR grade) were purchased from Beijing Chemical Reagent Company (Beijing, China). Concanavalin A, Roswell Park Memorial Institute (RPMI) 1640 medium, Dulbecco's modified Eagle medium (DMEM) medium, and fetal bovine serum (FBS) were supplied by Gibco (Grand Island, NY, USA). Alexa 635-phalloidin and Lysol-Tracker probes were purchased from Invitrogen (Grand Island, NY, USA). FITC was obtained from Sigma-Aldrich. Fluorochrome-labeled α-MHCI, α-MHC II, α-CD86, α-CD80, α-CD11c, α-CD69, α-CD44, α-CD62L, α-CD4, α-CD8 and α-CD19 antibodies were supplied by eBioscience (San Diego, CA, USA). Recombinant mouse GM-CSF and IL-4 were obtained from Peprotech (Rocky Hill, NJ, USA). Mouse cytokine ELISA kits were purchased from eBioscience. OVA derived (H-2 Kb, SIINFEKL) specific MHC I pentamers was purchased from ProImmune (Oxford, UK). p38 MAPK inhibitor SB203580 was purchased from Cell Signaling Technology (Danvers, MA, USA).
Han S., Ma W., Jiang D., Sutherlin L., Zhang J., Lu Y., Huo N., Chen Z., Engle J.W., Wang Y., Xu X., Kang L., Cai W, & Wang L. (2021). Intracellular signaling pathway in dendritic cells and antigen transport pathway in vivo mediated by an OVA@DDAB/PLGA nano-vaccine. Journal of Nanobiotechnology, 19, 394.
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4

Multiparametric Flow Cytometry Analysis

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Cells were labeled with fluorescence antibodies for mouse α-CD4, α-CD8, α-INF-γ, α-IL-2, α-IL-10, α-IL-4, α-IL-17A, α-CD44, and α-CD62L (eBioscience, CA). To assess immune responses after transplantation, total splenocytes or isolated CD4+ T cells were seeded on 48 well plates and stimulated in complete media for 4 hrs at 37°C with phorbol 12-myristate 13-acetate (PMA; 50 ng/ml; Sigma-Aldrich), Ionomycin (500ng/ml; Sigma-Aldrich) and Brefeldin A (eBioscience). Thereafter, cells were fixed, permeabilized and stained with respective antibodies at a concentration of 1–5 μg per 106 cells. Flow cytometry measurements were performed on a FACS Canto II (BD Bioscience, CA, USA) and data were analyzed using FlowJo (FlowJo Software, OR, USA).
Krenzien F., Quante M., Heinbokel T., Seyda M., Minami K., Uehara H., Biefer H.R., Schuitenmaker J.M., Gabardi S., Splith K., Schmelzle M., Petrides A.K., Azuma H., Pratschke J., Li X.C., ElKhal A, & Tullius S.G. (2016). Age-dependent metabolic and immunosuppressive effects of Tacrolimus. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 17(5), 1242-1254.
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