Fetal bovine serum catalog no a3160601

Cytokines human IFNα (Catalog No. 11200-2), monkey IFNα (Catalog No. 14110-1), mouse IFNα (Catalog No. 12100-1), humanIL-12 (Catalog No. 219-IL), monkey IL-12 (Catalog No. 3216-RL), dog IL-12 (Catalog No. 2118-CL), mouse IL-12 (Catalog No. 419-ML), human IL-15 (Catalog No. 247-IL) were obtained from R&D Systems (Minneapolis, MN, USA). Dog IFNα (Catalog No. AB1697202) was purchased from Abcam (Cambridge, UK). Antibodies specific to phosphorylated signal transducer and active of transcription proteins (pSTATs) were supplied by BD Biosciences (San Jose, CA, USA): Anti-pSTAT1-AF488 (Catalog No. 612596); Anti-pSTAT3-AF647 (Catalog No. 557815); Anti-pSTAT4-AF647 (Catalog No. 558137); Anti-pSTAT5-AF647 (Catalog No. 612599). Phosflow Lyse/Fix Buffer 5×(Catalog No. 558049) and BD Pharm Lyse lysing buffer (Catalog No. 555899) were purchased from BD Biosciences. Fetal bovine serum (Catalog No. A3160601) was purchased from Thermo Fisher Scientific (Waltham, MA, USA) and sodium azide (Catalog No. S8032) was obtained from Sigma Aldrich (St. Louis, MO, USA). D-PBS (Catalog No. 14190) and RPMI1640 medium (Catalog No. 11875-093) were obtained from Invitrogen (Grand Island, NY, USA).

Phosflow Lyse/Fix Buffer 5X (Catalog No. 558049) was purchased from BD Biosciences. Fetal bovine serum (Catalog No. A3160601) was purchased from Thermo Fisher Scientific and sodium azide (Catalog No. S8032) was obtained from Sigma Aldrich. D‐PBS (without Ca²⁺ or Mg²⁺) was obtained from Invitrogen (Catalog No. 14190). Glutathione S‐transferase (GST)‐tagged recombinant human kinase domains of JAK1, JAK2, and JAK3 were purchased from Thermo Fisher Scientific. His‐tagged recombinant human TYK2 kinase domain was expressed in SF21/baculovirus and purified using a two‐step affinity (Ni‐nitrilotriacetic acid) and size‐exclusion (SEC S200) purification method.