J. gendarussa leaves were collected from Pacet, Purwodadi, Surabaya, Gempol, Makassar, and Cibodas between September 2012 to January 2013 (
Table 1). Samples were properly authenticated by Department of Pharmacognosy and Phytochemistry, Airlangga University, Surabaya. Mature, dark green leaves of five different plants were collected from each location in triplicate; the leaves were air-dried and powdered. Moisture contents (MC) of the samples were 9.6 ± 1.7%, n = 105 (by using
Moisture Analyzer HB43-S, Mettler Toledo). The maximum permitted level of MC of the herbal medicine was 12%, w/w [31 ].
Soil collection was performed by using composite sampling. Fifteen sub-samples were collected randomly 6–8 inches from the surface [32 ].
Methanol,
2-propanol, and
formic acid were analytical reagents from Merck (Darmstadt, Germany). Purified water was from Sigma-Aldrich (St. Louis, MO, USA),
acetic acid from J.T. Baker (Phillipsburg, NJ, USA), and
NaOH from Agilent (Agilent solution for HPCE). All samples were filtered through a 0.2 µm Agilent Econofilter PVDF 13m.
Ningsih I.Y., Purwanti D.I., Wongso S., Prajogo B.E, & Indrayanto G. (2015). Metabolite Profiling of Justicia gendarussa Burm. f. Leaves Using UPLC-UHR-QTOF-MS. Scientia Pharmaceutica, 83(3), 489-500.