Pmh 100vs

Manufactured by Med Associates

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The PMH-100VS is a laboratory equipment product. It serves as a core function device for laboratory operations.

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Lab products found in correlation

Env 008ct, by Med Associates (7 mentions)

7 protocols using pmh 100vs

Operant Conditioning Chamber Setup

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Experimental procedures were conducted in 10 conditioning chambers (ENV-008CT; Med Associates, Georgia, VT), measuring 30.5 × 24.1 × 21.0 cm, enclosed in sound-attenuating cubicles. Each chamber had a variable-speed syringe pump (PMH-100VS; Med-Associates) located outside the cubicle. Tygon^® tubing was threaded from the pump syringe through a leash into the chamber to be attached to the catheter port. A recessed receptacle (5.2 × 5.2 × 3.8 cm) was centered on one sidewall of each chamber. A dipper arm could be raised to provide access to 0.1 ml of 26% (w/v) sucrose in this recessed receptacle. Two retractable levers were located on each side of the receptacle. A white cue-light (2.54 cm diameter; 28 V, 100-mA) was mounted 7 cm above each lever and a house-light (two white 28 V, 100-mA lamps) was located in the cubicle, 10 cm above the chamber ceiling. An infrared emitter/detector unit was located 4 cm above the stainless steel rod floor and 14.5 cm from the side wall containing the receptacle. The number of times this beam was broken provided a measure of chamber activity.

Pittenger S.T., Schaal V.L., Moore D., Guda R.S., Koul S., Yelamanchili S.V., Bevins R.A, & Pendyala G. (2018). MicroRNA cluster miR199a/214 are differentially expressed in female and male rats following nicotine self-administration. Scientific Reports, 8, 17464.

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Operant Conditioning Chamber for Drug Administration

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Conditioning chambers purchased from Med Associates (ENV-008CT; Georgia, VT, USA) were utilized for all behavioral testing. Chambers measured 30.5 × 24.1 × 21 cm and were enclosed in a sound-attenuating cubicle. A variable-speed syringe pump (PMH-100VS; Med-Associates) was located outside each cubicle. Tygon^® tubing was threaded from the pump syringe, through a leash, into the chamber to be attached to the catheter port that exited below the scapula of the rat. A recessed receptacle (5.2×5.2×3.8 cm) was centered on 1 sidewall of each chamber. A dipper arm, when raised, provided access to 0.1 ml of 26% (w/v) sucrose in this recessed receptacle. The chambers were also fitted with 2 retractable levers; 1 located on each side of the receptacle. A white cue-light (2.54 cm diameter; 28V, 100-mA) was mounted 7 cm above each lever. A house-light (two white 28V, 100-mA lamps) located 10 cm above the Perspex chamber ceiling was also in the cubicle.

Pittenger S.T., Chou S., Barrett S.T., Catalano I., Lydiatt M, & Bevins R.A. (2017). Nicotine- and cocaine-triggered methamphetamine reinstatement in female and male Sprague-Dawley rats. Pharmacology, biochemistry, and behavior, 159, 69-75.

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Operant Conditioning Chamber Setup for Rat Experiments

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For the present study, we used 10 conditioning chambers (ENV-008CT; Med Associates, Georgia, VT, USA) enclosed in sound-attenuating cubicles. Chambers measured 30.5×24.1×21.0 cm. A variable-speed syringe pump (PMH-100VS; Med-Associates) was located outside each cubicle. Tygon^® tubing was threaded from the pump syringe, through a leash, into the chamber where it was to be attached to the catheter port on the back of the rat. A recessed receptacle (5.2×5.2×3.8 cm) was centered on one sidewall of each chamber. A dipper arm, when raised, provided access to 0.1 ml of 26% (w/v) sucrose in this recessed receptacle. Two retractable levers were located on each side of the receptacle. A white cue light (2.54-cm diameter; 28V, 100-mA) was mounted 7 cm above each lever. A house light (two white 28V, 100-mA lamps) was located in the cubicle, 10 cm above the Perspex chamber ceiling. An infrared emitter/detector unit was located 4 cm above the rod floor and 14.5 cm from the side wall containing the receptacle. The number of times this beam was broken provided a measure of chamber activity.

Pittenger S.T., Barrett S.T., Chou S, & Bevins R.A. (2015). The effects of varenicline on methamphetamine self-administration and drug-primed reinstatement in female rats. Behavioural brain research, 300, 150-159.

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Behavioral Testing in Rodent Chambers

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Behavioral testing was conducted in chambers purchased from Med Associates (ENV-008CT; Georgia, VT, USA). Each chamber measured 30.5 × 24.1 × 21 cm and was enclosed in a sound-attenuating cubicle. A variable-speed syringe pump (PMH-100VS; Med-Associates) was located outside each cubicle. Tygon® tubing was threaded from the pump syringe, through a leash, into the chamber to be attached to the catheter port that exited below the scapula of the rat. A recessed receptacle (5.2 × 5.2 × 3.8 cm) was centered on one sidewall of each chamber. A dipper arm, when raised, provided access to 0.1 ml of 26% (w/v) sucrose in this recessed receptacle. A retractable lever was located on each side of the receptacle. A white cue-light (2.54 cm diameter; 28 V, 100-mA) was mounted 7 cm above each lever. A house-light (two white 28 V, 100-mA lamps) was located in the cubicle, 10 cm above the Perspex chamber ceiling.

Pittenger S.T., Chou S., Murawski N.J., Barrett S.T., Loh O., Duque J.F., Li M, & Bevins R.A. (2021). Female rats display higher methamphetamine-primed reinstatement and c-Fos immunoreactivity than male rats. Pharmacology, biochemistry, and behavior, 201, 173089.

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Operant Conditioning Chambers for Rodent Studies

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Experimental sessions were conducted in ten standard conditioning chambers (ENV‐008CT; Med Associates IV, Georgia, VT, USA). Chambers (30.5 cm × 24.1 cm × 21 cm; l × w × h) were light‐ and sound‐attenuating with aluminum sidewalls, metal rod floors, and clear polycarbonate ceiling, front, and back walls. The dipper receptacle was mounted on the right aluminum sidewall occupying a 5.2 × 5.2 × 3.8 cm (l × w × h) recessed space. Here, a dipper arm with an attached 0.1‐ml cup was raised to provide access to a solution inside the chamber. An infrared beam located within the dipper receptacle recorded the number of dipper entries. A variable‐speed syringe pump (PMH‐100VS; Med‐Associates) was located outside each cubicle. Tygon tubing was attached to the pump syringe, threaded through a metal spring leash, and into the ceiling of the chamber where it was to be attached to the catheter port on the back of the rat. Two retractable levers were located on each side of the receptacle. A white cue light (2.54‐cm diameter; 28 V, 100‐mA) was mounted 7 cm above each lever. A house light (two white 28 V, 100‐mA lamps) was located in the cubicle, 10 cm above the chamber ceiling. Med Associates interface and software (Med‐PC for Windows, version IV) were used to collect data and present programmed events. All Med‐PC programs are available upon request.

Chand S., Gowen A., Savine M., Moore D., Clark A., Huynh W., Wu N., Odegaard K., Weyrich L., Bevins R.A., Fox H.S., Pendyala G, & Yelamanchili S.V. (2021). A comprehensive study to delineate the role of an extracellular vesicle‐associated microRNA‐29a in chronic methamphetamine use disorder. Journal of Extracellular Vesicles, 10(14), e12177.

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Rat Operant Conditioning Chamber Setup

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Ten conditioning chambers (ENV-008CT; Med Associates, Georgia, VT, USA) measuring 30.5×24.1×21.0 cm and enclosed in sound-attenuating cubicles were utilized in this study. A variable-speed syringe pump (PMH-100VS; Med-Associates) was located outside each cubicle. A catheter port on the back of the rat was attached to the pump syringe via Tygon^® tubing threaded through a leash and out of the cubicle. A recessed receptacle (5.2×5.2×3.8 cm) fitted with a dipper arm (0.1 ml) was centered on one sidewall of each chamber. Two retractable levers were located on each side of the receptacle. A white cue light (2.54-cm diameter; 28V, 100-mA) was mounted 7 cm above each lever and a house light (two white 28V, 100-mA lamps; 10 cm above chamber ceiling) was located in the cubicle. An infrared emitter/detector unit was located 4 cm above the rod floor and 14.5 cm from the side wall containing the receptacle. Chamber activity was measure by the number of times this beam was broken.

Pittenger S.T., Barrett S.T., Chou S, & Bevins R.A. (2016). The effects of varenicline on methamphetamine self-administration and drug-primed reinstatement in male rats. Behavioural brain research, 320, 195-199.

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Sucrose Self-Administration Conditioning Chambers

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Conditioning sessions were conducted in 10 chambers (ENV-008CT; Med
Associates, Georgia, VT, USA). Each chamber measured
30.5×24.1×21 cm and was enclosed in a sound-attenuating cubicle.
A variable-speed syringe pump (PMH-100VS; Med-Associates) was located outside
each cubicle. Tygon^® tubing was threaded from the pump
syringe, through a leash, into the chamber to be attached to the catheter port
that exited below the scapula of the rat. A recessed receptacle
(5.2×5.2×3.8 cm) was centered on 1 sidewall of each chamber. A
dipper arm, when raised, provided access to 0.1 ml of 26% (w/v) sucrose
in this recessed receptacle. A retractable lever was located on each side of the
receptacle. A white cue-light (2.54 cm diameter; 28V, 100-mA) was mounted 7 cm
above each lever. A house-light (two white 28V, 100-mA lamps) was located in the
cubicle, 10 cm above the Perspex chamber ceiling. An infrared emitter/detector
unit was located 4 cm above the rod floor and 14.5 cm from the side wall
containing the receptacle. Chamber activity was defined as the number of times
this beam was broken.

Pittenger S.T., Swalve N., Chou S., Smith M.D., Hoonakker A.J., Pudiak C.M., Fleckenstein A.E., Hanson G.R, & Bevins R.A. (2016). Sex differences in neurotensin and substance P following nicotine self-administration in rats. Synapse (New York, N.Y.), 70(8), 336-346.

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