Polyclonal antibodies against lc3 l7543

Sunitinib (ST; S126061) was purchased from Aladdin (Seattle, WA, USA). 3-methyladenine (3-MA; M9281), chloroquine diphosphate salt (CQ; C6628), U0126 monoethanolate (u120), AICAR (A9978) and polyclonal antibodies against LC3 (L7543) were acquired from Sigma-Aldrich (St. Louis, MO, USA). H₂O₂ (E882) was purchased from Amresco (WA, USA). The antibodies against PARP- (9542), Phospho-AMPKα (Thr172; 2535), AMPKα (2532), Phospho-p44/42MAPK (ERK1/2; Thr202/Tyr204; 9106), Phospho-Acetyl-CoA Carboxylase (Ser79; 3661), p44/42 MAPK (total-ERK1/2; 9102) were obtained from Cell Signaling Technology (Boston, MA, USA). The antibody against LC3 (M152-3) for immunoprecipitation was purchased from Medical & Biological Laboratories (Naka-ku, Nagoya, Japan). The antibody against p62 (18420-1-AP) was bought from Proteintech (Wuhan, Hubei, China). The antibody against actin (TA-09) was acquired from ZhongShanJinQiao Biocompany (Beijing, China). MTS reagent powder (G1111) was obtained from Promega Corporation (Madision, WI, USA). Alexa Fluor 594 goat anti-rabbit IgG (H + L) (R37117) and Alexa Fluor 488 goat anti-mouse IgG (H + L) (A-11001) were purchased from Molecular Probes (Eugene, OR, USA).

Chloroquine diphosphate salt (CQ, C6628), MG132 (M8699) and polyclonal antibodies against LC3 (L7543) were purchased form Sigma-Aldrich (St. Louis, MO, USA). Antibodies against PARP (9542) and p44/42 MAPK (total-Erk1/2, 9102) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies of Ub (sc-8017) and p62 (sc-28359) were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against actin (TA-09) was obtained from ZhongShanJinQiao Biocompany (Beijing, China). MTS reagent powder (G1111) was acquired from Promega Corporation (Madison, WI, USA).