Omcl ac160ts w2

NP alone and NP-UAP56 complex were subjected to AFM analysis in air at room temperature (Bruker Nanoscope VIII, Bruker). The system was operated in tapping mode with a 100-μm scanner. Probes made of a single silicon crystal with a cantilever length of 129 µm and spring constant of 33–62 N/m (OMCL-AC160TS-W2, Olympus) were used for imaging. Data were collected in the height mode. Images were captured in 512 × 512 pixels and the captured images were flattened and plane-fitted before analysis. The size of the particles was calculated with a correction for tip effect as previously reported^{19 (link)}.

Peptide solutions were characterized using a Nano-Scope IIIa scanning probe work station equipped with a MultiMode head using a vertical engage E-series piezoceramic scanner (Veeco, Santa Barbara, CA). AFM probes were single-crystal silicon microcantilevers with 300-kHz resonant frequency and 42 Newton/meter spring constant model OMCL-AC160TS-W2 (Olympus). A 10μl of 0.1M NaOH was spotted onto mica, rinsed with 2 drops of deionized H₂O, then a 10-μl sample solution of PrP_106-126 or PrP-FL (From a 20μM stock solution) were spotted on freshly cleaved mica, incubated at room temperature for 3 minutes, rinsed with 20μl of filtered (Whatman Anotop 10) MilliQ water (Millipore), and blown dry with tetrafluoroethane (CleanTex MicroDuster III). Image data were acquired at scan rates between 1 and 2 Hz with drive amplitude and contact force kept to a minimum. Data were processed to remove vertical offset between scan lines by applying zero order flattening polynomials using Nanoscope software (Version 5.31r1,Veeco).