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Cortisol eia kit

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The Cortisol EIA kit is a laboratory equipment product designed to quantitatively measure the levels of the hormone cortisol in biological samples. It utilizes an enzyme-linked immunosorbent assay (EIA) technique to detect and quantify the presence of cortisol in the sample.

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Lab products found in correlation

Ethyl acetate, by Merck Group (1 mentions) Vibra cell vcx130, by Sonics (1 mentions) Ethyl ether, by Thermo Fisher Scientific (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Clariostar, by BMG Labtech (1 mentions) Protein assay kit, by Bio-Rad (1 mentions) Aldosterone eia kit, by Cayman Chemical (1 mentions)

Spelling variants of this product

EIA kit (244 citations) Cortisol (5 citations) Cortisol Express EIA Kit (4 citations)

16 protocols using cortisol eia kit

1

Measuring Cortisol and CRH Levels

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Cortisol levels and corticotropin-releasing hormone (CRH) in serum samples were measured using the commercial Cortisol EIA kit (Cayman Chemical Company, Ann Arbor, MI, USA), and the CRH Enzyme-linked Immunosorbent Assay Kit (USCN Life Science Inc., Wuhan, China), respectively, following the manufacturer's protocols.
Shin H., Choi S.J., Cho A.R., Kim D.Y., Kim K.H, & Kwon O. (2016). Acute Stress-Induced Changes in Follicular Dermal Papilla Cells and Mobilization of Mast Cells: Implications for Hair Growth. Annals of Dermatology, 28(5), 600-606.
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2

Cortisol Quantification in Embryos

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Embryos (n=30) were collected at 30hpf and homogenized in 100μL of ultrapure water by electric pestle. Ethyl acetate (500μl; Sigma Aldrich, St. Louis MO) was added to the lysate, centrifuged, and the insoluble fraction discarded. The soluble fraction was decanted, dehydrated, and re-suspended in 110μl EIA buffer; total cortisol was measured using a Cortisol EIA kit (Cayman Chemical, Ann Arbor MI) according to manufacturer's instructions.
Kwan W., Cortes M., Frost I., Esain V., Theodore L.N., Liu S.Y., Budrow N., Goessling W, & North T.E. (2016). Central Nervous System Regulation of Embryonic HSPC Production via Stress-Responsive Glucocorticoid Receptor Signaling. Cell stem cell, 19(3), 370-382.
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3

Measuring Cortisol in Medaka Larvae

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Steroid hormones in XX medaka larvae (5 pooled fishes) at 0 days post-hatching (dph) (stage 39) were extracted with diethyl ether as previously described (11 (link), 31 (link)). Cortisol levels were measured using a Cortisol EIA kit (Cayman Chemical, Ann Arbor, MI) in accordance with the manufacturer's instructions.
Uchimura T., Hara S., Yazawa T., Kamei Y, & Kitano T. (2019). Involvement of Heat Shock Proteins on the Transcriptional Regulation of Corticotropin-Releasing Hormone in Medaka. Frontiers in Endocrinology, 10, 529.
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4

Quantifying Cortisol Levels in Samples

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Samples frozen in 800 μL of phosphate-buffered saline (PBS) were thawed, and homogenized on ice for 90 s with a Vibra-Cell VCX 130 (Sonics and Materials Inc.) ultrasonic processor. The cortisol was extracted from 640 μL of the resulting homogenate three times using 3 mL of ethyl ether (Fisher Scientific), which was then evaporated off by placing the test tubes in a 42 °C water bath for approximately 12–18 h. The samples were then reconstituted with 500 μL of PBS at 4 °C for approximately 30 h, vortexing occasionally. The cortisol in each sample was quantified using a Cortisol EIA Kit (Cayman Chemical). The cortisol levels were normalized to the amount of protein in each of the samples, which was determined by following the microplate procedure of a Pierce BCA Protein Assay Kit (Thermo Scientific) using 25 μL aliquots of either bovine serum albumin standards or sample homogenate for the unknowns. The cortisol extraction efficiency was calculated to be approximately ~70 %, which is similar to previously published results (Alsop & Vijayan, 2008 ). The calculated extraction efficiency was used to correct all recorded cortisol values to the actual amount of cortisol per sample.
Krug RG I.I., Poshusta T.L., Skuster K.J., Berg M.R., Gardner S.L, & Clark K.J. (2014). A transgenic zebrafish model for monitoring glucocorticoid receptor activity. Genes, brain, and behavior, 13(5), 478-487.
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5

Serum Cortisol ELISA Quantification

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Serum cortisol was determined using ELISA kit (Commercially available Cortisol EIA kit, catalogue no. 500360, Cayman Chemicals, USA). The assay was performed as per instruction provided with the kit using ELISA plate reader (Biotek India Pvt. Ltd.).
Kumar N., Thorat S.T., Kochewad S.A, & Reddy K.S. (2024). Manganese nutrient mitigates ammonia, arsenic toxicity and high temperature stress using gene regulation via NFkB mechanism in fish. Scientific Reports, 14, 1273.
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6

Serum Cortisol and HSP 70 Evaluation

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Serum cortisol and HSP 70 were determined using ELISA kit (Commercially available Cortisol EIA kit, catalogue no. 500360, Cayman Chemicals, USA). Similarly, HSP 70 was also determined through EIA kit (catalogue no. EKS-700B, Bioguenix/Enzo Life Science, Mumbai, India). The assay was performed as per instruction provided with the kit. The final reading was obtained at 420 nm using ELISA plate reader (Clario Star, BMG Labtech, Germany).
Kumar N., Gupta S.K., Chandan N.K., Bhushan S., Singh D.K., Kumar P., Kumar P., Wakchaure G.C, & Singh N.P. (2020). Mitigation potential of selenium nanoparticles and riboflavin against arsenic and elevated temperature stress in Pangasianodon hypophthalmus. Scientific Reports, 10, 17883.
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7

Aldosterone and Cortisol Response in H295R Cells

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H295R cells were plated to 60% confluence in 24‐multiwell plates. Thereafter, they were exposed to either 100 mg·dL−1d‐glu, 450 mg·dL−1d‐glu, 100 mg·dL−1d‐glu plus 100 nmol·L−1 AII (for aldosterone), or 450 mg·dL−1d‐glu plus 100 nmol·L−1 AII (for aldosterone) for 72 h. The aldosterone and cortisol concentrations of the media were thereafter measured by Aldosterone EIA Kit and Cortisol EIA Kit (Cayman Chemical), respectively, after their extraction with dichloromethane according to the manufacturer's instructions. The obtained data were normalized by the protein concentrations measured by Protein Assay Kit (Bio‐Rad).
Shimada H., Kogure N., Noro E., Kudo M., Sugawara K., Sato I., Shimizu K., Kobayashi M., Suzuki D., Parvin R., Saito‐Ito T., Uruno A., Saito‐Hakoda A., Rainey W.E., Ito S., Yokoyama A, & Sugawara A. (2017). High glucose stimulates expression of aldosterone synthase (CYP11B2) and secretion of aldosterone in human adrenal cells. FEBS Open Bio, 7(9), 1410-1421.
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8

Serum Cortisol and HSP-70 Expression in Fish

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Cortisol was quantified in the serum of fish from the different experimental groups through ELISA. The quantification was done using a commercially available Cortisol EIA kit (Catalog no. 500360), procured from Cayman Chemicals, USA. The assay was performed according to the protocol provided along with the kit. The absorbance was read in the ELISA plate reader (Biotek India Pvt. Ltd.). The expression of HSP-70 in gill and liver was determined as per the manufacturer’s instructions (Bioguenix/Enzo Life Science, Mumbai, India). The absorbance was read in the ELISA plate reader (Biotek India Pvt. Ltd.).
Kumar N., Thorat S.T, & Reddy K.S. (2023). Multi biomarker approach to assess manganese and manganese nanoparticles toxicity in Pangasianodon hypophthalmus. Scientific Reports, 13, 8505.
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9

Cortisol Measurement in Serum Samples

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A plastic syringe was used to collect blood from the caudal vasculature of the SW and FW groups (day 1, day 7 and day 30, n = 8 in each group) in Eppendorf tubes. Centrifugation at 8000× g for 5 min at 4 °C yielded serum that was then stored in a freezer until further use. The cortisol EIA kit (Cayman Chemical, Ann Arbor, MI, USA) was used to measure cortisol levels. Three milliliters of diethyl ether was used to extract the serum samples. The supernatant was maintained at 40 °C in a water bath for evaporation of ether after extraction and then stored at a freezer until further analysis. Then, cortisol extracts were re-suspended in PBS buffer. We established a parallelism between the standard curve and a serial dilution of the extracted solution. The cortisol concentrations (ng/mL) in the blood could then be calculated.
Aruna A., Lin C.J., Nagarajan G, & Chang C.F. (2021). Neurohypophysial Hormones Associated with Osmotic Challenges in the Brain and Pituitary of the Euryhaline Black Porgy, Acanthopagrus schlegelii. Cells, 10(11), 3086.
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10

Measuring Plasma Corticosterone and Skin Analysis

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Blood was collected from each mouse in the evening, and the plasma corticosterone level was measured using a Cortisol EIA kit (Cayman Chemical Company, Ann Arbor, MI, USA). Skin samples were cut into small pieces, sonicated in 1% triton PBS, centrifuged for 20 min, and the supernatants were collected for analysis.
Terao M., Tani M., Itoi S., Yoshimura T., Hamasaki T., Murota H, & Katayama I. (2014). 11β-Hydroxysteroid Dehydrogenase 1 Specific Inhibitor Increased Dermal Collagen Content and Promotes Fibroblast Proliferation. PLoS ONE, 9(3), e93051.
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