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Nanog homeobox nanog

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Nanog homeobox (Nanog) is a key transcription factor involved in the regulation of embryonic stem cell self-renewal and pluripotency. It plays a critical role in maintaining the undifferentiated state of embryonic stem cells.

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Lab products found in correlation

Tra 1 60, by Merck Group (2 mentions) Tra 1 81, by Merck Group (2 mentions) Octamer binding transcription factor 4 oct4, by Cell Signaling Technology (2 mentions) Stage specific embryonic antigen 4 ssea, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

NANOG (54 citations)

2 protocols using nanog homeobox nanog

1

Induced Pluripotent Stem Cell Generation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Induced pluripotent stem cells (iPSCs) were generated as previously described (Holler et al., 2016 (link)). In brief, early passage fibroblast (Fig. S2C) using the following markers: Octamer-binding transcription factor 4 (Oct4; 1:400; Cell Signaling Technologies); Stage-Specific Embryonic Antigen 4 (SSEA; 1:500; Cell Signaling Technologies); Nanog homeobox (Nanog; 1:200; R&D Systems); Sex Determining Region Y-Box 2 (Sox2;1:200: ThermoScientific); Tra1–60 and Tra1–81 (1:150 each; Millipore). Correct chromosomal numbers were checked by karyotyping at WiCell (Madison, Wisconsin, USA; Fig. S2D).
Bakkar N., Starr A., Rabichow B.E., Lorenzini I., McEachin Z.T., Kraft R., Chaung M., Macklin-Isquierdo S., Wingfield T., Carhart B., Zahler N., Chang W.H., Bassell G.J., Betourne A., Boulis N., Alworth S.V., Ichida J.K., August P.R., Zarnescu D.C., Sattler R, & Bowser R. (2020). The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease. Neurobiology of disease, 149, 105228.
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2

Induced Pluripotent Stem Cell Generation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Induced pluripotent stem cells (iPSCs) were generated as previously described (Holler et al., 2016 (link)). In brief, early passage fibroblast (Fig. S2C) using the following markers: Octamer-binding transcription factor 4 (Oct4; 1:400; Cell Signaling Technologies); Stage-Specific Embryonic Antigen 4 (SSEA; 1:500; Cell Signaling Technologies); Nanog homeobox (Nanog; 1:200; R&D Systems); Sex Determining Region Y-Box 2 (Sox2;1:200: ThermoScientific); Tra1–60 and Tra1–81 (1:150 each; Millipore). Correct chromosomal numbers were checked by karyotyping at WiCell (Madison, Wisconsin, USA; Fig. S2D).
Bakkar N., Starr A., Rabichow B.E., Lorenzini I., McEachin Z.T., Kraft R., Chaung M., Macklin-Isquierdo S., Wingfield T., Carhart B., Zahler N., Chang W.H., Bassell G.J., Betourne A., Boulis N., Alworth S.V., Ichida J.K., August P.R., Zarnescu D.C., Sattler R, & Bowser R. (2020). The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease. Neurobiology of disease, 149, 105228.
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