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2 protocols using yoda1

1

Osteogenic Differentiation Assays

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SB203580 (p38 inhibitor), and a kit for enzyme activity staining of ALP were purchased from WAKO (Osaka, Japan). The Alizarin Red staining kit was obtained from PG Research (Tokyo, Japan). The Oil Red O stainning kit was purchased from Sigma-Aldorich (MO, USA). GsMTx4 and Yoda1 were purchased from Abcam (Tokyo, Japan) and Tocris Bioscience (Bristol, UK), respectively. Recombinant murine noggin was obtained from PeproTech (NJ, USA). U0126 was purchased from Cell Signaling (MA, USA).
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2

Mechanobiology of Cellular Signaling Pathways

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The antibodies against E-cadherin (catalog no. 3159); N-cadherin (catalog no. 13116); vimentin (catalog no. 5741); YAP (catalog no. 14074); p-YAP (catalog no. 13008); Smad 2/3 (catalog no. 8685); HIF-1α (catalog no. 36169); LATS 1 (catalog no. 3477); p-LATS 1 (catalog no. 13008); survivin (catalog no. 2808); CTGF (catalog no. 86641); and CYR61 (catalog no. 14479) were purchased from Cell Signaling Technology (CST); antibody against p-Smad 2/3 (catalog no. ab272332) was purchased from Abcam; and antibody against Piezo 1 (catalog no. NBP1-78537) was purchased from Novus Biologicals. Verteporfin (YAP inhibitor, catalog no. HY-B0146) was purchased from MedChemExpress; GsMTx4 (Piezo 1 inhibitor, catalog no. ab141871) was purchased from Abcam. Yoda 1 (Piezo 1 agonist, catalog no. ab141871) was purchased from Abcam.
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