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Confocal software lcs 1537

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Leica Confocal Software LCS-1537 is a software application developed for the control and operation of Leica's confocal microscopy systems. The software is designed to capture, analyze, and process images acquired using Leica's confocal microscopes.

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Lab products found in correlation

Sp2 confocal microscope, by Leica (2 mentions) Asp175, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

LCS software (22 citations) Leica Confocal Software LCS (9 citations) Leica Confocal Software (LCS) version 2.61 Build 1537 (3 citations)

2 protocols using confocal software lcs 1537

1

Gentamicin-Induced Apoptosis in RPTECs

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RPTECs were grown on sterile glass coverslips and treated with gentamicin with or without cilastatin for 24 h. Cells were incubated with antibody against cleaved caspase-3 (Asp175; 1:50; Cell Signaling Technology, Inc., Beverly, MA, USA). Immunolocalization was examined with the 20× PL-APO 0.7 numerical aperture objective of a Leica-SP2 confocal microscope (Leica Microsystems, Heidelberg, Germany). The staining analysis was performed with Leica Confocal Software LCS-1537 (Leica Microsystems).
Jado J.C., Humanes B., González-Nicolás M.Á., Camaño S., Lara J.M., López B., Cercenado E., García-Bordas J., Tejedor A, & Lázaro A. (2020). Nephroprotective Effect of Cilastatin against Gentamicin-Induced Renal Injury In Vitro and In Vivo without Altering Its Bactericidal Efficiency. Antioxidants, 9(9), 821.
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2

TUNEL Assay for Apoptosis Detection in Kidney Tissue

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Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) in paraffin-embedded kidney tissue sections was performed using the in situ Fluorescein FragEL DNA Fragmentation Detection Kit (Calbiochem, San Diego, CA, USA) following the manufacturer's protocol. The TUNEL-positive cells were visualized with a Leica-SP2 confocal microscope (Leica Microsystems, Heidelberg, Germany). Various measurements of positive staining fluorescence were taken using Leica confocal software LCS-1537 (Leica Microsystems) in 10 nonoverlapping random fields viewed at ×20 magnification.
Lázaro A., Humanes B., Jado J.C., Mojena M., González-Nicolás M.Á., del Cañizo J.F., Tejedor A, & Lledó-García E. (2016). Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study. BioMed Research International, 2016, 2518626.
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