Cocl2 powder

BV2 microglia cells were obtained from GuangZhou Jennio Bio- tech. The cells were cultured in DMEM (Sigma–Aldrich, Shanghai, China) with 10% fetal bovine serum (Gibco, Life technologies, USA), 100 units/ml penicillin (sigma) and 100 μg/ml streptomycin (sigma) in an incubator containing 5% CO₂ at 37°C. BV2 cells were sub-cultured when reaching 90% confluence. The eighth passage was used in the present study.
Propofol (Sigma, St. Louis, MO, USA), calcium chelator BAPTA-AM (Sigma, St. Louis, MO, USA), CAMKIIα inhibitor KN93 (Sigma, St. Louis, MO, USA), ERK inhibitor U0126 and JAK1 inhibitor INCB039110 were dissolved in DMSO (Sigma, St. Louis, MO, USA). The final concentration of DMSO was adjusted to 0.01% for each solution to avoid possible nonspecific effects. A 500mM stock solution of cobalt chloride (CoCl₂) was prepared by dissolving CoCl₂ powder (Sigma-Aldrich, Shanghai, China) in serum-free DMEM.

HT22 cells were obtained from GuangZhou Jennio Bio- tech and maintained in DMEM (HyClone Laboratories, Logan, Utah, USA) with 5 mM glucose and 10% fetal bovine serum. Cells were incubated in a humidified atmosphere with 5% CO₂ at 37 °C and sub-cultured when reaching 90% confluence. The eighth passage was used in the present study.
Propofol (Sigma, St. Louis, MO, USA), PP2A inhibitor okadaic acid (Sigma, St. Louis, MO, USA), and PP2A activator FTY720 (Sigma, St. Louis, MO, USA) were dissolved in DMSO (Sigma, St. Louis, MO, USA). In order to avoid possible nonspecific effects, the final concentration of DMSO was adjusted to 0.01% for each solution. A 500 mM stock solution of CoCl₂ was prepared by dissolving CoCl₂ powder (Sigma-Aldrich, Dorset, UK) in serum-free DMEM.