Forty-eight C57BL/6JRj mice (24 eight-week-old males and 24 eight-week-old females) were purchased from a verified international breeder (Janvier Labs, Le Genest-Saint-Isle, France). All mice were raised following the recommendations of the Federation of Laboratory Animal Science Associations (FELASA). The mice were randomly housed in groups of four in type II long filter-top cages (Tecniplast, Buguggiate, Italy; SealSafe Plus, polyphenylsulfone, 36.5 cm × 21 cm × 14 cm), equipped with red transparent shelters, cocoons, and opaque 10 cm PVC tubes (tunnels) to transport the mice in an animal-friendly way. Housing followed a 12/12 light–dark cycle (200 lux from 7:00 to 19:00) in a temperature and humidity-controlled animal room (22–24 °C and 50–55%, respectively). Water and food (ssniff M-Z Extrudat, ssniff, Soest, Germany) were provided ad libitum. The mice were kept in same-sex groups of four. All animals were allowed a habituation period of one week before the experimental phase began.
M z extrudat
Manufactured by Ssniff
Sourced in Germany
The M-Z Extrudat is a laboratory equipment used for the extrusion of materials. It is designed to process and shape various materials through the application of pressure and heat. The core function of the M-Z Extrudat is to produce consistent and uniform extruded samples for further analysis and testing.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using m z extrudat
1
Comprehensive Mouse Housing and Acclimation Protocol
2
Timed Pregnant C57BL/6JRj Mouse Study
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We performed the study according to the guidelines of the German Animal Welfare Act and the European Directive 2010/63/EU for the protection of animals used for scientific purposes. Reporting was carried out according to the ARRIVE guidelines for reporting in vivo experiments [24 (link)]. The timed pregnant C57BL/6JRj mice (specific pathogen-free [SPF], tested according to the recommendations of the Federation of Laboratory Animal Science Associations [FELASA]), were purchased on gestation day (12–14) from a registered international breeder (Janvier Labs, Le Genest-Saint-Isle, France). After arrival, the mice were housed in type II long filter top cages (Tecniplast, Buguggiate, Italy; SealSafe Plus, polyphenylsulfone, 365 mm L x 207 mm W x 140 mm H, Greenline). The mice were kept in a 12:12-h light / dark cycle (lights 06:00–18:00) in a temperature and humidity-controlled animal room (22 ± 2°C, 55 ± 5%). Food (ssniff M-Z Extrudat, ssniff, Soest, Germany) and water were supplied ad libitum.
3
Timed Pregnancy in C57BL/6J Mice
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Primiparous timed-pregnant C57BL/6J mice (SPF, specific-pathogen-free, tested according to the FELASA, Federation for Laboratory Animal Science Associations, recommendations 29 ) were purchased on gestation day 14 from a registered international breeder (Janvier Labs, Le Genest-Saint-Isle, France), where they were kept in type II cages (330 cm 2 ) with 100 g of bedding material (spruce, Rettenmaier, Rosenberg, Germany) enriched with nest-building material (7-8 g sizzle nest per two pregnant females). A successful mating is indicated by a vaginal plug. The day the plug was detected was considered day 0.
On arrival at our facility, all dams were single housed in type II long, filter-top cages (Tecniplast, Buguggiate, Italy; SealSafe Plus, polyphenylsulfone, 365 mm L Â 207 mm W Â 140 mm H Greenline) with varying enrichment described in experimental design. The mice were maintained under a 12:12-h light/dark cycle (lights on 06:00-18:00) in a temperature-and humidity-controlled animal room (22 AE 2 C, 55 AE 5%). Food (ssniff M-Z Extrudat, ssniff, Soest, Germany) and water were supplied ad libitum.
On arrival at our facility, all dams were single housed in type II long, filter-top cages (Tecniplast, Buguggiate, Italy; SealSafe Plus, polyphenylsulfone, 365 mm L Â 207 mm W Â 140 mm H Greenline) with varying enrichment described in experimental design. The mice were maintained under a 12:12-h light/dark cycle (lights on 06:00-18:00) in a temperature-and humidity-controlled animal room (22 AE 2 C, 55 AE 5%). Food (ssniff M-Z Extrudat, ssniff, Soest, Germany) and water were supplied ad libitum.
4
Transgenic Mouse Model of NAFLD
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Transgenic mice, 22-weeks old, with NAFLD due to adipose tissue-specific overexpression of sterol regulatory-element binding protein-1c (SREBP-1c) and matched C57B16 controls were maintained under pathogen-free conditions on a 12-h light-dark cycle, received standardized diet (Ssniff M-Z Extrudat, 4.5% fat; SSNIFF Spezialdiäten GmbH, Soest, Germany) and water ad libitum. Mice were exsanguinated through cervical incision and sacrificed by cervical dislocation. Organs were snap frozen in liquid nitrogen for future analyses. All experiments were performed according to the guidelines for the care and use of animals (GV-SOLAS [Society for Laboratory Animal Science]) and approved by the local council of animal care in line with the requirements of the German Animal Protection Act.
5
Postnatal Mouse Microstructure Analysis
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This study was carried out according to the German Animal Welfare Act guidelines and the European Directive 2010/63/EU for protecting animals used for scientific purposes. Reporting was carried out following the ARRIVE guidelines [31 ]. The offspring (postnatal 5 days—P5) were sacrificed by expert personnel for scientific use according to §4 of the Animal Welfare Act, legalized and controlled by the Animal Welfare Authority of the Medical University.
Term-pregnant C57BL/6JRj mice were purchased (Janvier Labs, Le Genest-Saint-Isle, France). After arrival, the mice were housed in type II long filter-top cages (SealSafe Plus, polyphenyl sulfone, 365 mm L × 207 mm W × 140 mm H, Greenline; Tecniplast, Buguggiate, Italy). The mice were kept in a 12:12 h light/dark cycle (lights 06:00–18:00) in a temperature- and humidity-controlled animal room (22 ± 2 °C, 55 ± 5%). Food (ssniff M-Z Extrudat, ssniff, Soest, Germany) and water were supplied ad libitum.
The pup was sacrificed with sodium pentobarbital (i.p. 0.1 mL/10 g BW, Narcoren® (16 g pentobarbital sodium 16.0 g, benzyl alcohol 3.0 g; Boehringer Ingelheim, Ingelheim, Germany)) at P5 for micro-computed tomography.
Term-pregnant C57BL/6JRj mice were purchased (Janvier Labs, Le Genest-Saint-Isle, France). After arrival, the mice were housed in type II long filter-top cages (SealSafe Plus, polyphenyl sulfone, 365 mm L × 207 mm W × 140 mm H, Greenline; Tecniplast, Buguggiate, Italy). The mice were kept in a 12:12 h light/dark cycle (lights 06:00–18:00) in a temperature- and humidity-controlled animal room (22 ± 2 °C, 55 ± 5%). Food (ssniff M-Z Extrudat, ssniff, Soest, Germany) and water were supplied ad libitum.
The pup was sacrificed with sodium pentobarbital (i.p. 0.1 mL/10 g BW, Narcoren® (16 g pentobarbital sodium 16.0 g, benzyl alcohol 3.0 g; Boehringer Ingelheim, Ingelheim, Germany)) at P5 for micro-computed tomography.
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