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Cell scraper

Manufactured by Starlab
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The cell scraper is a laboratory tool used to detach adherent cells from the surface of a cell culture vessel. It features a flat, flexible blade that can be used to gently scrape and lift cells from the growth surface without damaging the cells.

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Lab products found in correlation

Tri reagent, by Starlab (2 mentions) Realplex software, by Eppendorf (2 mentions) Chloroform, by Merck Group (2 mentions) Luna universal qpcr master mix, by New England Biolabs (2 mentions)

3 protocols using cell scraper

1

RNA Extraction and RT-qPCR Analysis

Cited 1 time
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RNA extraction and cDNA synthesis from fungal cells was done using TRI reagent (LabConsulting) exactly as described previously (Tscherner et al., 2012 (link)). For RNA isolation from BMDMs challenged with C. albicans at a MOI of 5:1 (fungi to BMDMs), 1 mL of TRI reagent was added per 35 mm dish (containing 1×106 BMDMs), collected using a cell scraper (Starlab) and transferred into 1.5 mL tube on ice. After centrifugation at 14,000 × g for 15 min at 4°C, the supernatant was transferred into a fresh tube containing 200 μl chloroform (Sigma-Aldrich). Further steps and cDNA synthesis were done exactly as for fungal cells (Tscherner et al., 2012 (link)).
Gene transcription analysis from cDNA samples was done by quantitative real-time PCR (qPCR) using the Luna Universal qPCR Master Mix (New England Biolabs). Amplification curves were analyzed using the Realplex Software (Eppendorf) and relative quantification of qPCR products was done using the efficiency corrected ΔΔCt method (Pfaffl, 2001 (link)). RIP1 was used as a reference gene for fungal gene transcription analysis (Hnisz et al., 2010 (link)) and Actb for BMDMs, peritoneal cells and kidney samples. See Table S1 for all oligos used in this study.
Jenull S., Mair T., Tscherner M., Penninger P., Zwolanek F., Silao F.G., de San Vicente K.M., Riedelberger M., Bandari N.C., Shivarathri R., Petryshyn A., Chauhan N., Zacchi L.F., LeibundGut -Landmann S., Ljungdahl P.O, & Kuchler K. (2021). The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence. Cell reports, 36(3), 109406.
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2

Standardizing Candida Albicans Biofilm Assays

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All C. albicans clinical isolates (n = 42) were standardised to 1 × 106 cells/mL in RPMI-1640 and biofilms grown in flat-bottomed 96 well microtitre plates at 37°C for 24 h and biomass of each isolate assessed using the crystal violet (cv) assay as previously reported [17 (link)], and isolates were grouped based on their level of biomass distribution (OD570nm values). Isolates that fell below the 1st quartile (Q1) were classed as having low biofilm formation (LBF), strains with a biomass greater than the 3rd quartile (Q3) were deemed isolates with high biofilm formation (HBF), and those that lay in between were classified as intermediate biofilm formation (IBF Q2). C. albicans biomass was further assessed using dry weight measurements. Selected isolates with LBF and HBF were grown as biofilms in 12 well tissue culture plates for 24 h, as previously described, and the resulting biomass homogenised in 1 mL of PBS using a cell scraper (STARLAB, Milton Keynes, UK). This was then passed through a 0.22 μm filter disc (Satorius Stedim) using a vacuum and filters were dried at 40°C overnight before measuring each isolates dry weight. Uninoculated controls were used for background correction.
Sherry L., Rajendran R., Lappin D.F., Borghi E., Perdoni F., Falleni M., Tosi D., Smith K., Williams C., Jones B., Nile C.J, & Ramage G. (2014). Biofilms formed by Candida albicans bloodstream isolates display phenotypic and transcriptional heterogeneity that are associated with resistance and pathogenicity. BMC Microbiology, 14, 182.
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3

RNA Extraction and RT-qPCR Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA extraction and cDNA synthesis from fungal cells was done using TRI reagent (LabConsulting) exactly as described previously (Tscherner et al., 2012 (link)). For RNA isolation from BMDMs challenged with C. albicans at a MOI of 5:1 (fungi to BMDMs), 1 mL of TRI reagent was added per 35 mm dish (containing 1×106 BMDMs), collected using a cell scraper (Starlab) and transferred into 1.5 mL tube on ice. After centrifugation at 14,000 × g for 15 min at 4°C, the supernatant was transferred into a fresh tube containing 200 μl chloroform (Sigma-Aldrich). Further steps and cDNA synthesis were done exactly as for fungal cells (Tscherner et al., 2012 (link)).
Gene transcription analysis from cDNA samples was done by quantitative real-time PCR (qPCR) using the Luna Universal qPCR Master Mix (New England Biolabs). Amplification curves were analyzed using the Realplex Software (Eppendorf) and relative quantification of qPCR products was done using the efficiency corrected ΔΔCt method (Pfaffl, 2001 (link)). RIP1 was used as a reference gene for fungal gene transcription analysis (Hnisz et al., 2010 (link)) and Actb for BMDMs, peritoneal cells and kidney samples. See Table S1 for all oligos used in this study.
Jenull S., Mair T., Tscherner M., Penninger P., Zwolanek F., Silao F.G., de San Vicente K.M., Riedelberger M., Bandari N.C., Shivarathri R., Petryshyn A., Chauhan N., Zacchi L.F., LeibundGut -Landmann S., Ljungdahl P.O, & Kuchler K. (2021). The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence. Cell reports, 36(3), 109406.
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