Emulsion PCR was performed according to the emPCR Amplification Method Manual—LIB-A of Roche GS Junior+ Series (Roche, Mannheim, Germany). Preparation of samples for the Roche 454 sequencing was also performed according to the Sequencing Manual—LIB-A of Roche GS Junior+ Series protocol (Roche, Mannheim, Germany), and long-range sequencing was run on Roche 454 Junior or FLX instruments. An average of 450 MHC class I reads was obtained for each animal.
+ Open protocol