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Agilent 4x180 k exon arrays

Manufactured by Agilent Technologies
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The Agilent 4x180 K Exon arrays are a high-density microarray platform designed for transcriptome analysis. The arrays contain probes targeting over 180,000 exons, allowing for comprehensive coverage of the human transcriptome. The arrays are formatted in a 4-plex layout, enabling the analysis of multiple samples in a single experiment.

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Lab products found in correlation

Rna 6000 pico kit, by Agilent Technologies (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Rna clean up and concentration micro kit, by Norgen Biotek (2 mentions)

Spelling variants of this product

Agilent arrays (7 citations) Exon Array (2 citations)

2 protocols using agilent 4x180 k exon arrays

1

Profiling LARP1-Bound RNA Transcripts

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RNA was immunoprecipitated with 15-μg LARP1 polyclonal antibody (SDIX) or IgG isotype control (CST) following the method described by Keene et al.42 (link) RNA was extracted with Trizol (Life Technologies, Carlsbad, CA, USA) and purified with RNA clean-up and concentration micro kit (Norgen Biotek, Thorold, ON, Canada). Input and immunoprecipitated RNA obtained from four independent experiments were analyzed in duplicate on Agilent exon arrays by Oxford Gene Technology (Begbroke, UK). RNA was quality assessed in a bioanalyser with the RNA 6000 pico kit (Agilent Technologies, Santa Clara, CA, USA), labeled with Agilent low-input QuickAmp WT labeling kit (Agilent Technologies) and hybridized to the Agilent 4x180 K Exon arrays (Agilent Technologies) as per manufacturer protocols.
Mura M., Hopkins T.G., Michael T., Abd-Latip N., Weir J., Aboagye E., Mauri F., Jameson C., Sturge J., Gabra H., Bushell M., Willis A.E., Curry E, & Blagden S.P. (2014). LARP1 post-transcriptionally regulates mTOR and contributes to cancer progression. Oncogene, 34(39), 5025-5036.
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2

LARP1-Bound RNA Profiling

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RNA was immunoprecipitated with 15μg LARP1 polyclonal antibody (SDIX) or IgG isotype control (CST) following the method described by Keene et al19 (link). RNA was extracted with Trizol (Life Technologies) and purified with RNA clean-up and concentration micro kit (Norgen Biotek). Input and immunoprecipitated RNA obtained from 4 independent experiments were analyzed in duplicate on Agilent exon arrays by Oxford Gene Technology. RNA was quality assessed in a bioanalyser with the RNA 6000 pico kit (Agilent Technology), labeled with Agilent Low-Input QuickAmp WT (LIQA WT) labeling Kit (Agilent Technology) and hybridized to the Agilent 4X 180K Exon arrays as per manufacturer protocols.
Mura M., Hopkins T.G., Michael T., Abd-Latip N., Weir J., Aboagye E., Mauri F., Jameson C., Sturge J., Gabra H., Bushell M., Willis A.E., Curry E, & Blagden S.P. (2014). LARP1 post-transcriptionally regulates mTOR and contributes to cancer progression. Oncogene, 34(39), 5025-5036.
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