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Rabbit anti α tubulin antibody

Manufactured by Proteintech
Sourced in United States

Rabbit anti‐α‐tubulin antibody is a primary antibody that specifically binds to the alpha subunit of the tubulin protein. Tubulin is a key structural component of microtubules, which are critical for cellular processes such as cell division, intracellular transport, and cell motility. This antibody can be used in various applications, such as immunofluorescence, immunohistochemistry, and Western blotting, to detect and visualize the α‐tubulin protein.

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2 protocols using rabbit anti α tubulin antibody

1

Arginase-1 Protein Expression Assay

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Phenol red‐impregnated thread (Zone‐Quick) was purchased from Ayumi Pharmaceutical Co. (Tokyo, Japan). Medetomidine (Dorbene) was purchased from Kyoritsu Seiyaku Co. (Tokyo, Japan), midazolam from Sandoz K.K. (Tokyo, Japan), butorphanol (Vetorphale) from Meiji Seika Pharma Co., Ltd (Tokyo, Japan), paper plugs (JM paper point) from J. Morita Corp. (Osaka, Japan), Tissue Total RNA Purification Mini Kit from Favorgen (Ping‐Tung, Taiwan), DNase I from Nippon Gene (Tokyo, Japan), ReverTra Ace qPCR RT Master Mix from Toyobo (Tokyo, Japan), SYBR Premix Ex Taq II (TB Green Premix Ex Taq II Tli RNaseH Plus) and EmeraldAmp PCR Master Mix from Takara Bio Inc. (Shiga, Japan), RIPA lysis buffer (EzRIPA Lysis kit) from Atto Co. (Tokyo, Japan), skim milk from Morinaga‐Nyugyo (Tokyo, Japan), rabbit anti‐arginase 1 antibody from GeneTex, Inc. (Irvine, CA, USA), rabbit anti‐α‐tubulin antibody from Proteintech (Rosemont, IL, USA), anti‐rabbit IgG HRP‐linked antibody from Beckman Coulter (Fullerton, CA, USA), whole‐cell lysates of 293T cells, which overexpress human liver arginase (arginase 1), from Abcam (Cambridge, UK), ECL Western blotting detection reagents from GE Healthcare (Chicago, IL, USA), and CB‐1158 dihydrochloride from MedChemExpress Co., Ltd (Monmouth Junction, NJ, USA).
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2

Autophagy Regulation Mechanisms Elucidation

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Bafilomycin A1 (Baf A1, 20 μM) was purchased from Selleck (USA), and the classical autophagy inhibitor 3-methyladenine (3-MA, 1 mM) was purchased from Sigma-Aldrich (USA). Protein A/G-Sepharose (Santa Cruz, USA) was used in the immunoprecipitation assays. The antibodies used in this study included rabbit anti-MPR (cation independent) antibody (Abcam, USA), rabbit anti-α-Tubulin antibody (Proteintech, USA), rabbit anti-cathepsin B antibody (Santa Cruz, USA), mouse anti-cathepsin D antibody (Santa Cruz, USA), rabbit anti-Lamp1 antibody (Abcam, USA), rat anti-Lamp1 antibody (Abcam, USA), mouse anti-Golgin 160 antibody (Santa Cruz, USA), mouse anti-EEA1 antibody (Santa Cruz, USA), mouse anti-GLUT4 antibody (Santa Cruz, USA), mouse anti-TBC1D5 antibody (Santa Cruz, USA), mouse anti-VPS35 antibody (Santa Cruz, USA), mouse anti-VPS29 antibody (Santa Cruz, USA), mouse anti-SNX1 antibody (Santa Cruz, USA), mouse anti-SNX2 antibody (Santa Cruz, USA), mouse anti-SNX5 antibody (Santa Cruz, USA), mouse anti-SNX6 antibody (Santa Cruz, USA), mouse anti-Rab7 antibody (Abcam, USA), HRP-conjugated mouse anti-Actin antibody (Proteintech, USA), mouse anti-α-Tubulin antibody (Proteintech, USA), rabbit anti-ATG5 antibody (Cell Signaling Technology, USA) and rabbit anti-Dynactin p150glued antibody (Cell Signaling Technology, USA).
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