Nih rodent diet 31 open formula

The animal experiments were conducted according to a protocol approved by the Animal Research Advisory Committee of the NIH (RBB-159-2SA) in accordance with the National Institutes of Health Guidelines for Animal Research. Female athymic nude mice weighing approximately 26 g were supplied by the Frederick Cancer Research Center, Animal Production (Frederick, MD) and housed with ad libitum access to NIH Rodent Diet #31 Open Formula (Envigo) and water on a 12 hr light/dark cycle. Xenografts were generated by the subcutaneous injection of 3 × 10⁶ MIA PaCa-2 (America Type Cell Collection (ATCC), Manassas, VA) or Hs 766T (Threshold Pharmaceuticals, Redwood City, CA) pancreatic ductal adenocarcinoma cells. (Kim et al., 2009 (link)) Both cell lines were tested in May 2013 and authenticated by IDEXX RADIL (Columbia, MO) using a panel of microsatellite markers. Molecular testing of cell lines for multiple pathogens, including mycoplasma, was performed at the time of receipt and prior to in vivo studies. All cell lines were maintained in RPMI 1640 supplemented with 10% fetal calf serum and antibiotics.

The animal experiments were conducted according to a protocol approved by the Animal Research Advisory Committee of the NIH (RBB-159-2SA) in accordance with the National Institutes of Health Guidelines for Animal Research. Female athymic nude mice weighing approximately 26 g were supplied by the Frederick Cancer Research Center, Animal Production (Frederick, MD) and housed with ad libitum access to NIH Rodent Diet #31 Open Formula (Envigo) and water on a 12-hour light/dark cycle. Xenografts were generated by the subcutaneous injection of 3 × 10⁶ MiaPaCa-2 (America Type Cell Collection (ATCC), Manassas, VA, USA) or Hs766t (Threshold Pharmaceuticals, Redwood City, CA, USA) pancreatic ductal adenocarcinoma cells. Both cell lines were tested in May 2013 and authenticated by IDEXX RADIL (Columbia, MO) using a panel of microsatellite markers