Serum 25-hydroxyvitamin D [25(OH)D] concentration was measured by liquid chromatography tandem mass spectrometry [22 ] with local interassay precision of 7.3 %. Biomarkers of bone formation [serum levels of osteocalcin (OC) and bone-specific alkaline phosphatase (BSAP)] and bone resorption [serum C-telopeptides (CTx)] were also measured. OC was measured by ELISA (ALPCO Diagnostics, Salem, NH; %CV 5.0–6.5 %); CTx was measured by immunoradiometric assay (Immunodiagnostic Systems, Tyne and Wear, UK; %CV 5.2–6.8 %); and BSAP was measured by access chemiluminescent immunoassay (Beckman Coulter, Brea, CA; %CV 1.5–2.6 %). Samples were batched to minimize chance of batch effects.
Immunoradiometric assay
Manufactured by Immunodiagnostic Systems
Sourced in United Kingdom
Immunoradiometric assay (IRMA) is an analytical technique used for the quantitative measurement of specific analytes in a sample. The assay utilizes radioisotope-labeled antibodies to detect and quantify the target analyte. IRMA provides a sensitive and accurate method for the determination of various biomolecules, such as hormones, proteins, and other substances, in biological samples.
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Lab products found in correlation
2 protocols using immunoradiometric assay
1
Vitamin D and Bone Biomarkers Measurement
2
Bone Turnover Markers in Clinical Trials
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Assays for bone turnover markers and parathyroid hormone (PTH) were performed in batch at the end of the study. Fasting serum levels of type 1 cross-linked C-telopeptide (CTX), a bone resorption marker, were measured by immunoradiometric assay (Immunodiagnostic Systems, Fountain Hills, AZ), with intra- and inter-assay CV of 5.2–6.8% and 5.6–7.4%. Fasting serum procollagen type 1 N-terminal propeptide (P1NP), a bone formation marker, was measured by RIA (Orion Diagnostica, Finland), with intra- and inter-assay CV of 3.5–5.3% and 3.6–5.4%. Serum PTH was measured by chemiluminescent immunoassay (Beckman Coulter, Fullerton, CA) with an intra- and inter-assay CV of 1.6–2.6% and 2.8–5.8%. Additional labs were measured in clinical laboratories using standard clinical assays (i.e. calcium, creatinine: spectrophotometry; 25-hydroxyvitamin D: chemiluminescent immunoassay). Hypocalcemia was defined as <8.6 mg/dL, as per the normal range of our clinical lab assay.
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