Racemic standards of N‐hexanoyl‐homoserine lactone (HHL), N‐heptanoyl‐homoserine lactone (HpHL), N‐octanoyl‐homoserine lactone (OHL), N‐decanoyl‐homoserine lactone (DHL), and N‐dodecanoyl‐homoserine lactone (dDHL), N‐(3‐oxohexanoyl)‐homoserine lactone (OHHL), M9 broth, d ‐(+)‐glucose BioReagent grade, magnesium sulfate BioReagent grade, dl ‐methionine, and all HPLC grade solvents were obtained from Sigma‐Aldrich, (St. Louis, Missouri, USA). N,O‐bis(trimethylsilyl)trifluoroacetamide (BSTFA) with 1% v/v trimethylchlorosilane (TMCS), 1 gram SupelTM‐select HLB SPE (HLB, hydrophilic‐lipophilic balance) cartridges, and β‐DEX™ 225 (25% 2,3‐di‐O‐acetyl‐6‐O‐TBDMS‐β‐cyclodextrin in SPB‐20 poly(20% phenyl/80% dimethylsiloxane) column, 30 m x 0.25 mm i.d. (film thickness 0.25 μm) were obtained from Millipore Sigma (Supelco, Bellefonte, Pennsylvania, USA). Photobacterium broth was obtained from Carolina Biological (Burlington, North Carolina, USA). Deionized water was obtained from an in‐house Millipore system producing water of 18.2 MΩ•cm resistivity.
N decanoyl homoserine lactone
Manufactured by Merck Group
Sourced in United States
N‐decanoyl‐homoserine lactone is a chemical compound used as a laboratory reagent. It is a bacterial signaling molecule that plays a role in quorum sensing, a process by which bacteria can coordinate their behavior. This compound can be used in research applications to study bacterial communication and population dynamics.
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Lab products found in correlation
Dl methionine, by Merck Group (1 mentions)
M9 broth, by Merck Group (1 mentions)
C6 hsl, by Cayman Chemical (1 mentions)
3 oxo c10 hsl, by Merck Group (1 mentions)
C10 hsl, by Merck Group (1 mentions)
N 3 oxo dodecanoyl l homoserine lactone, by Merck Group (1 mentions)
C4 hsl, by Cayman Chemical (1 mentions)
N 3 oxo hexanoyl l homoserine lactone, by Merck Group (1 mentions)
3 oxo c6 hsl, by Merck Group (1 mentions)
3 oxo c12 hsl, by Merck Group (1 mentions)
2 protocols using n decanoyl homoserine lactone
1
Quantification of Acyl-Homoserine Lactones
2
Degradation of Quorum Sensing Signals by Bacillus spp.
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The AHLs degradation activity of cell-free lysates of Bacillus spp. isolates 30b, 32C, and 6 was assessed against different AHL signals using the well diffusion assay. AHL signals tested were; 100 µM C4-HSL (Cayman), 50 µM C6-HSL, 50 µM N- decanoyl-homoserine lactone (C10-HSL), 25 µM N-(3-oxohexanoyl)-L-homoserine lactone (3-oxo-C6-HSL), 25 µM N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C10-HSL), 25 µM 3-oxo-C12-HSL (Sigma-Aldrich). The relative degradation activity was calculated for each AHL signal by calculating the residual AHL from a calibration curve constructed using different concentrations of standard AHL and their induced zone diameter using two AHLs reporter strains; Chromobacterium violaceum (C. violaceum) CV026 and A. tumafaciens KYC55, which respond to short chain and medium-to-long chain AHLs, respectively.39 (link)–42 The maximum degrading activity is defined as 100% relative degradation activity.43 (link)
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