To determine and quantify Alpha‐KG in rice fermented liquid, capillary electrophoresis time‐of‐flight mass spectrometry (CE‐TOFMS) analysis was carried out using an Agilent G7100 CE system, an Agilent G6224AA LC/MSD TOF system, and an Agilent 1200 series isocratic HPLC pump. The system was controlled by Agilent ChemStation software for CE and MassHunter software for the Agilent TOFMS.
G7100 ce system
Manufactured by Agilent Technologies
Sourced in United States, Germany
The G7100 CE system is a capillary electrophoresis instrument designed for analytical separation and detection of various samples. The system features a high-performance detection module and an advanced automated sample handling mechanism. The core function of the G7100 CE system is to perform precise and reliable analytical separations.
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4 protocols using g7100 ce system
1
Alpha-KG Quantification in Rice Ferment
2
Comprehensive Analytical Techniques for Biosimilar Characterization
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SEC was performed on an Agilent 1200 HPLC system (Agilent Technologies, Waldbronn, Germany). RPLC was performed on an Agilent 1100 HPLC system for method evaluation (section 3.1 ) and on an Agilent 1200 HPLC system for biosimilar comparability exercises (section 3.2 ). Detection was carried out with a diode-array detector. SEC multiangle light scattering (MALS) was performed on a 1260 Bio-Inert HPLC coupled with a Bio-Dual Angle LS/DLS (Agilent). CGE-UV separations were carried out on a G7100 CE system (Agilent) coupled to a diode-array detector. For RPLC quadrupole time-of-flight (QTOF) experiments, a UHPLC system coupled to a DTIMS-QTOF mass spectrometer 6,560 (Agilent) was used. A Dual Agilent Jet Stream ESI was used as electrospray ionization source.
3
Metabolite Analysis by CE-MS and HPLC
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The dried extracts were dissolved in 20 μL Milli-Q water and analyzed using an Agilent G7100 CE system, an Agilent G6224AA LC/MSD time-of-flight (TOF) system, and an Agilent 1200 series isocratic high-performance liquid chromatography (HPLC) pump equipped with a 1:100 splitter for delivery of the sheath liquid (Agilent Technologies, Palo Alto, CA, USA). Agilent Chem-Station software for CE/MS and MassHunter software for TOFMS were used as the system control and data acquisition software. The CE/MS analytical conditions for cationic and anionic metabolite analyses were as described previously [49 (link)].
4
RPLC-Chip-DTIMS-QTOF Mass Spectrometry
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All CZE experiments were performed using a G7100 CE system (Agilent Technologies, Waldbronn, Germany) hyphenated with a 6560-hybrid DTIMS-QTOF (Agilent Technologies, Waldbronn, Germany) with detection in positive ionization mode for all acquisitions.
Hyphenation of both instruments was achieved by the use of a coaxial sheath liquid interface sprayer (Agilent Technologies). The sheath liquid consisted of MeOH/H2O/FA (80:20:0.5, v/v/v) and was pumped into the sprayer at a flow rate of 0.38 μL/min to guarantee stable spraying. The working conditions of RPLC-Chip are described in [7 (link)].
Hyphenation of both instruments was achieved by the use of a coaxial sheath liquid interface sprayer (Agilent Technologies). The sheath liquid consisted of MeOH/H2O/FA (80:20:0.5, v/v/v) and was pumped into the sprayer at a flow rate of 0.38 μL/min to guarantee stable spraying. The working conditions of RPLC-Chip are described in [7 (link)].
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