Apigenin (Api) (A3145) ≥97% (TLC) from parsley, 4-hydroxytamoxifen (4-OHT) (H7904) ≥98% Z isomer, and β-estradiol (E2) (E8875) ≥98% were purchased from Sigma-Aldrich (St Louis, MO, USA).
β estradiol e2 e8875
Manufactured by Merck Group
Sourced in United States
β-estradiol (E2) (E8875) is a chemical compound that is commonly used as a reference standard in laboratory research. It is a naturally occurring steroid hormone that plays a crucial role in the female reproductive system. The core function of this product is to serve as a reference material for analytical and biological experiments.
Automatically generated - may contain errors
3 protocols using β estradiol e2 e8875
1
Apigenin, Tamoxifen, and Estradiol Protocols
2
MCF-7 Breast Cancer Cell Culture and Hormone Starvation
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MCF-7 breast cancer cells were routinely grown in DMEM/nutrient mixture F-12 supplemented with 10% FBS and 100 µg/ml of Primocin (InvivoGen), which is active against bacteria, mycoplasma, and fungi. Cells were routinely checked for mycoplasma infection. For the experiments, cells were plated in the same media at a density of 2 × 105 cells in 12-well plates, and for 24 h, they were hormone starved by washing them thoroughly with PBS, adding DMEM/F-12 media without phenol red, and supplementing with 2% dextran-coated, charcoal-treated FBS. 72 h later, cells were treated with β-estradiol (E2, E8875; Sigma-Aldrich) or vehicle for the indicated time and harvested for downstream applications.
HEK293 cells were routinely grown in DMEM supplemented with 10% FBS, 100 U/ml of penicillin, and 100 µg/ml of streptomycin.
HEK293 cells were routinely grown in DMEM supplemented with 10% FBS, 100 U/ml of penicillin, and 100 µg/ml of streptomycin.
3
Modulating Inflammatory Signaling Pathways
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Human IL-6 Recombinant Protein (RP-8619; Invitrogen, Waltham, MA, USA) and bovine serum albumin (BSA; J10857-36; Thermo Scientific, Waltham, MA, USA) were dissolved in double-distilled water. STAT3 Inhibitor VI S3I-201 (sc-204304; Santa Cruz Biotechnology, Dallas, TX, USA) was dissolved in DMSO. Tocilizumab (TCZ; Actemra®; Roche, Switzerland) was used and diluted in saline. β-Estradiol (E2; E8875; Sigma, St. Louis, MO, USA) was dissolved in 100% ethanol. NF-κB inhibitor p-XSC (ab142600; Abcam, Cambridge, UK) was dissolved in DMSO. Tamoxifen (TAM/4-OHT; H6278; Sigma, St. Louis, MO, USA) was dissolved into 100% ethanol (EtOH).
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