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2 protocols using tropine

1

Dopamine Transporter Binding Assay

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Neurobasal-A medium (no glucose, no sodium pyruvate), B-27 supplement, and B-27 supplement minus antioxidants were purchased from Life Technologies. Cocaine hydrochloride, atropine, benzoic acid, benztropine, ecgonine, lidocaine, scopolamine, and glutathione agarose were purchased from Sigma-Aldrich. Anisodamine, benzocaine, benzoylecgonine, benztropine, 3-p-FBT (hydrochloride), procaine, and tropine were purchased from Cayman Chemical. Protein A/G PLUS-agarose was purchased from Santa Cruz Biotechnology. [3H]cocaine was purchased from PerkinElmer Life Sciences. FLAG-DAT plasmid (pcihygroflagsynhDAT) was a gift from Jonathan Javitch, Columbia University, New York, NY (Addgene plasmid #19990) (77 (link)). PDGF-TMD-GFP plasmid (pFU-no toxin-PE) was a gift from Ines Ibañez-Tallon, Rockefeller University, New York, NY (Addgene plasmid #24149) (78 (link)).
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2

Neuronal Cell Culture Reagents

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Neurobasal-A medium (no glucose, no sodium pyruvate), B-27 supplement and B-27 supplement minus antioxidants were purchased from Life Technologies (Grand Island, NY). Cocaine hydrochloride, atropine, benzoic acid, benztropine, ecgonine, lidocaine, scopolamine and glutathione agarose were purchased from Sigma-Aldrich (St. Louis, MO). Anisodamine, benzocaine, benzoylecgonine, benztropine, 3-p-FBT (hydrochloride), procaine, and tropine were purchased from Cayman Chemical (Ann Arbor, Michigan). Protein A/G plus-agarose was purchased from Santa Cruz Biotechnology (Paso Robles, CA). [ 3 H]cocaine was purchased from PerkinElmer Life Sciences (Boston, MA). FLAG-DAT plasmid (pcihygroflagsynhDAT) was a gift from Jonathan Javitch, Columbia University (Addgene plasmid # 19990) 28 . PDGF-TMD-GFP plasmid (pFU-no toxin-PE) was a gift from Ines Ibanez-Tallon (Addgene plasmid # 24149) 29 .
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