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Dna methylation elisa kit

Manufactured by Cayman Chemical
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Sourced in United States

The DNA methylation ELISA kit is a laboratory instrument designed to quantify the level of DNA methylation in a given sample. It employs an enzyme-linked immunosorbent assay (ELISA) technique to detect and measure the presence of methylated DNA.

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Lab products found in correlation

Phosphodiesterase, by Merck Group (2 mentions) Genejet genomic dna purification kit, by Thermo Fisher Scientific (2 mentions) Nuclease p1, by Merck Group (2 mentions) Alkaline phosphatase, by Merck Group (2 mentions) Nanodrop machine, by Thermo Fisher Scientific (1 mentions)

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ELISAs

2 protocols using dna methylation elisa kit

1

DNA Methylation Quantification Protocol

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DNA was extracted from samples using the GeneJET Genomic DNA Purification Kit (Thermo Fisher, Suwanee, GA) following manufacturer's instructions. Thereafter, the isolated DNA was treated with nuclease P1, alkaline phosphatase, and phosphodiesterase (Sigma-Aldrich, St. Louis, MO) (27 (link)). CpG methylation of the samples was quantified with a DNA methylation ELISA kit (Cayman Chemical, Ann Arbor, Michigan) following manufacturer's instructions.
Korsmo H.W., Dave B., Trasino S., Saxena A., Liu J., Caviglia J.M., Edwards K., Dembitzer M., Sheeraz S., Khaldi S, & Jiang X. (2022). Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner. Frontiers in Nutrition, 9, 841787.
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2

DNA Methylation Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA was extracted from samples using the GeneJET Genomic DNA Purification Kit (Thermo Fisher, Suwanee, GA, USA) following the manufacturer’s instructions. DNA quantity and quality was determined by a Nanodrop machine (Thermo Fisher). An A260/280 ratio over 1.8 was considered acceptable. Thereafter, the isolated DNA was treated with nuclease P1, alkaline phosphatase, and phosphodiesterase (Sigma-Aldrich, St. Louis, MO, USA) [36 (link)]. CpG methylation of the samples was quantified with a DNA methylation ELISA kit (Cayman Chemical, Ann Arbor, MI, USA) following the manufacturer’s instructions.
Kadam I., Trasino S.E., Korsmo H., Lucas J., Pinkas M, & Jiang X. (2024). Prenatal Choline Supplementation Improves Glucose Tolerance and Reduces Liver Fat Accumulation in Mouse Offspring Exposed to Ethanol during the Prenatal and Postnatal Periods. Nutrients, 16(9), 1264.
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