Cyclosporine

Manufactured by Bio-Techne

Cyclosporine is a laboratory product used for immunosuppression research. It is a cyclic peptide compound that inhibits the activation and proliferation of T-cells. The core function of Cyclosporine is to modulate the immune response, making it a valuable tool for scientists studying immune system regulation and related biological processes.

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Lab products found in correlation

Capsazepine, by Bio-Techne (1 mentions) Capsaicin, by Bio-Techne (1 mentions) Thapsigargin, by Thermo Fisher Scientific (1 mentions) Guinea pig anti insulin, by Abcam (1 mentions) Mouse anti actin, by MP Biomedicals (1 mentions) Kn 93, by Bio-Techne (1 mentions) Alexa 594, by Thermo Fisher Scientific (1 mentions) Carbamoylcholine chloride carbachol, by Bio-Techne (1 mentions) Mouse anti calreticulin, by Enzo Life Sciences (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions)

2 protocols using cyclosporine

Synthesis and Stability of Peptide Constructs

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Capsaicin, capsazepine, and cyclosporine were purchased commercially (Tocris Bioscience). Peptides were synthesized using microwave chemistry on a Liberty microwave peptide synthesizer (CEM Corp).12 We called the peptides V1‐Cal and A5‐Cal. Peptides were synthesized as 1 polypeptide with transactivator of transcription (TAT)_47‐57 carrier in the following order: N‐terminus–TAT_47‐57–spacer (Gly‐Gly)–cargo–C terminus. Peptide stability was assessed using a peptidase assay (Data S1).

Hurt C.M., Lu Y., M. Stary C., Piplani H., Small B.A., Urban T.J., Qvit N., Gross G.J., Mochly‐Rosen D, & Gross E.R. (2016). Transient Receptor Potential Vanilloid 1 Regulates Mitochondrial Membrane Potential and Myocardial Reperfusion Injury. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(9), e003774.

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Antibody sources and reagents for TMEM24 study

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Antibodies were obtained from the following commercial sources: rabbit anti-TMEM24 (Aviva Biotechnology), mouse anti-Calreticulin (Enzo Life Science), rabbit anti-Proinsulin/Insulin, guinea pig anti-Insulin (AbCam), mouse anti-mRFP (Rockland) and mouse anti-Actin (MP Biomedical). Mouse anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was generated in our lab, while Rabbit anti-TMEM24 was generated by YenZym Antibodies LLC using a C-terminal peptide from TMEM24 with the following sequence: CLRSGTKLIFRRRPRQKE. Alexa488, Alexa594, and Alexa647 conjugated secondary antibodies and Thapsigargin were from Life Technologies; Bisindolylmaleimide II, KN-93, Cyclosporine and Carbamoylcholine chloride (Carbachol) were from Tocris. Lipids were from Avanti Polar Lipids and American Radiolabeled Chemicals.

Lees J.A., Messa M., Sun E.W., Wheeler H., Torta F., Wenk M.R., De Camilli P, & Reinisch K.M. (2017). Lipid transport by TMEM24 at ER–plasma membrane contacts regulates pulsatile insulin secretion. Science (New York, N.Y.), 355(6326), eaah6171.

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