Human and mice skin tissues were fixed with 4% paraformaldehyde in PBS, embedded in paraffin, sectioned, and stained with H&E for histopathologic examination. Immunohistochemistry was performed on formalin-fixed, paraffin-embedded skin tissues. The slices were probed overnight with SerpinB7 (Sigma-Aldrich, HPA024200), mKi67 (Abcam, ab16667). For Immunofluorescence, skin frozen sections were cryosectioned, blocked, and then stained with anti-Filaggrin (Biolegend, 905804), anti-KRT10 (Abcam, ab76318), anti-Loricrin (Biolegend, 905101). Images were captured using an Olympus BX600 microscope (Olympus Corporation, Tokyo, Japan) and SPOT Flex camera (Olympus Corporation, Tokyo, Japan) and were analyzed with ImagePro Plus (version 6.0, Media Cybernetics) software. The epithelial thickness and infiltrating cells were calculated in independent regions as described previously [47 (link)].
Anti filaggrin
Manufactured by BioLegend
Anti-Filaggrin is a lab equipment product that serves as a key component in research and analysis. It functions as a specific antibody that recognizes and binds to the filaggrin protein, a structural protein found in the epidermis. This product enables researchers to detect and study the presence and distribution of filaggrin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Mki67, by Abcam (1 mentions)
Bx600 microscope, by Olympus (1 mentions)
Anti krt10, by Abcam (1 mentions)
Anti loricrin, by BioLegend (1 mentions)
Spot flex camera, by Olympus (1 mentions)
Image pro plus, by Media Cybernetics (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti mmp 1, by Santa Cruz Biotechnology (1 mentions)
Imagequant las 500, by GE Healthcare (1 mentions)
Nupage 4 12 bis tris protein gel, by Thermo Fisher Scientific (1 mentions)
Pro prep protein extraction solution, by iNtRON Biotechnology (1 mentions)
2 protocols using anti filaggrin
1
Histopathological and Immunological Analysis of Skin Tissues
2
Evaluating the Effects of E. cava Extract on HaCaT Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HaCaT, human keratinocytes, were treated with SRM-1649b in presence or absence of E. cava extract for 48 h or 72 h. Following treatment, the cells were washed with cold PBS and lysed with Pro-prep™ protein extraction solution (iNtRON biotechnology). Quantification of protein was by BCA assay. Protein samples of equal quantity were loaded and then separated on NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo Scientific, Waltham, MA, USA) and transferred to PVDF membrane. The following antibodies were used for detection in western blotting: anti-filaggrin (Biolegend, PRB-417P), anti-β-actin (Santa Cruz, sc-47778), and anti-MMP-1 (Santa Cruz, sc-58377). Antibody binding on the membrane was visualized by ImageQuant™ LAS 500 (GE Healthcare Life Sciences). Each protein band was analyzed using image J and the protein levels normalized to β-actin on the same blot.
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