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Facsdiva 8.0.1 cell sorter

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The BD FACSDiva 8.0.1 is a cell sorter that is used for the detection, identification, and isolation of specific cell populations from complex samples. It is designed to provide highly accurate and efficient cell sorting capabilities.

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Lab products found in correlation

Linoleic acid, by Cayman Chemical (2 mentions) Oleic acid, by Cayman Chemical (2 mentions) Ril 2, by R&D Systems (2 mentions) Ril 7, by R&D Systems (2 mentions) Ril 33, by R&D Systems (2 mentions) Golgiplug, by BD (2 mentions)

2 protocols using facsdiva 8.0.1 cell sorter

1

Expansion and Stimulation of ILC2s from Murine Models

Cited 1 time
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Wt and Pla2g5-null mice received four doses of 25ug of Alternaria in 20ul of PBS i.n. on day 0, 3, 6 and 9 and euthanized 18h later in order to expand ILC2s prior to FACs sorting. Sorting of ILC2s (CD45+ Lin− (CD3, CD19, Ly6g, CD11c, CD11b, Nk1.1, FcεR1−), Thy1.2+) was performed using a FACSDiva 8.0.1 cell sorter (BD Bioscience). Purified CD45+ lin-Thy1.2+ cells (>98%) were rested for 40h with 10ng/mL rIL-2 and rIL-7 (R&D Systems, Minneapolis, MN) in 96 well around bottom plates (20000 cells per well). Prior to stimulation, the medium was changed to fresh medium. ILC2s were cultured with 30ng/mL rIL-33 (R&D Systems), 200 μM LinOleic Acid (Cayman Chemical) or 200 μM Oleic Acid (Cayman Chemical)22 (link) or all together for 8h. For intracellular cytokine staining, 1 μl/mL of Golgi Plug (BD Bioscience) was added to ILC2s 6h before collection for FACs analysis.
Yamaguchi M., Samuchiwal S.K., Quehenberger O., Boyce J.A, & Balestrieri B. (2017). Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms. Mucosal immunology, 11(3), 615-626.
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2

Expansion and Stimulation of ILC2s from Murine Models

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Wt and Pla2g5-null mice received four doses of 25ug of Alternaria in 20ul of PBS i.n. on day 0, 3, 6 and 9 and euthanized 18h later in order to expand ILC2s prior to FACs sorting. Sorting of ILC2s (CD45+ Lin− (CD3, CD19, Ly6g, CD11c, CD11b, Nk1.1, FcεR1−), Thy1.2+) was performed using a FACSDiva 8.0.1 cell sorter (BD Bioscience). Purified CD45+ lin-Thy1.2+ cells (>98%) were rested for 40h with 10ng/mL rIL-2 and rIL-7 (R&D Systems, Minneapolis, MN) in 96 well around bottom plates (20000 cells per well). Prior to stimulation, the medium was changed to fresh medium. ILC2s were cultured with 30ng/mL rIL-33 (R&D Systems), 200 μM LinOleic Acid (Cayman Chemical) or 200 μM Oleic Acid (Cayman Chemical)22 (link) or all together for 8h. For intracellular cytokine staining, 1 μl/mL of Golgi Plug (BD Bioscience) was added to ILC2s 6h before collection for FACs analysis.
Yamaguchi M., Samuchiwal S.K., Quehenberger O., Boyce J.A, & Balestrieri B. (2017). Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms. Mucosal immunology, 11(3), 615-626.
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