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Stratagene mx3005 p real time thermal cycler

Manufactured by Agilent Technologies
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The Stratagene Mx3005 P Real Time Thermal Cycler is a laboratory instrument designed for real-time polymerase chain reaction (PCR) analysis. It is capable of performing quantitative, qualitative, and multiplex PCR experiments.

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Lab products found in correlation

Primer premier v5, by Thermo Fisher Scientific (1 mentions) Sybr green, by Takara Bio (1 mentions) Vector nit v 11, by Thermo Fisher Scientific (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Rneasy mini column, by Qiagen (1 mentions)

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Stratagene MX3005 (3 citations) Thermal cycler (2 citations)

2 protocols using stratagene mx3005 p real time thermal cycler

1

Verification of RNA-Seq Results by qRT-PCR

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To verify the RNA-Seq results, quantitative RT-PCR was conducted using SYBR-green (TaKaRa Biotechnology Co., Ltd, Dalian, China) and Stratagene Mx3005 P Real Time Thermal Cycler (Agilent, America) with the following program: 95 °C for 30 s, followed by 40 cycles of 95 °C for 10 s, and then annealing at 65 °C–95 °C for 30 s. We used the Primer Premier v. 5.0 and Vector NIT v.11.0 software (Applied Biosystems) to design primers based on the sequences of key genes of interest identified in our library. The D. cambodiana actin gene was used as an internal control. The sequences of primers used in this study are provided in Table S6.
Zhu J.H., Cao T.J., Dai H.F., Li H.L., Guo D., Mei W.L, & Peng S.Q. (2016). De Novo transcriptome characterization of Dracaena cambodiana and analysis of genes involved in flavonoid accumulation during formation of dragon’s blood. Scientific Reports, 6, 38315.
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2

Quantitative Analysis of LRAT mRNA

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Total RNA from frozen tissues was extracted using TRIzol reagent (Invitrogen) and purified by the RNeasy mini columns (QIAgen). DNase-treated RNA concentration was measured using NanoDrop 1000 spectrophotometer (Thermo Scientific), and the quality was analyzed with Bioanalyzer 2100 (Agilent). cDNA was synthesized from 1 μg total RNA using multiscribe reverse transcriptase. LRAT RNA levels were measured using predeveloped LRAT TaqMan® assay (Hs00428109_m1, Applied Biosystems). The human GAPDH (Hs99999905_m1) gene was used for normalization. Quantitative PCR was performed with the Stratagene Mx3005p real-time thermal cycler (Agilent Technologies, Santa Clara, CA). Each sample was measured in triplicates, and the average Ct (threshold cycle) was used for calculating mRNA levels.
Cheng Y.W., Pincas H., Huang J., Zachariah E., Zeng Z., Notterman D.A., Paty P, & Barany F. (2014). High incidence of LRAT promoter hypermethylation in colorectal cancer correlates with tumor stage. Medical oncology (Northwood, London, England), 31(11), 254.
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