Glucose 6 phosphate dehydrogenase from baker s yeast

(±)-Doxazosin mesylate [(±)-DOX], (+)-doxazosin mesylate [(+)-DOX], and (−)-doxazosin mesylate [(−)-DOX] standards (>99.9% purity) were provided by the New Drug Research and Development Center of the North China Pharmaceutical Group Corporation (Shijiazhuang, China). β-Nicotinamide adenine dinucleotide phosphate hydrate (NADP), glucose-6-phosphate dehydrogenase from Baker’s yeast (S. cerevisiae), glucose-6-phosphate, prazosin (internal standard, IS), phenacetin, tolbutamide, and human CYP enzymes (CYP3A4, CYP2D6, CYP2C19, CYP2C8, CYP1A2, CYP2E1, and CYP2C9) were purchased from Sigma-Aldrich (St Louis, USA). Dextromethorphan hydrobromide, paclitaxel, and testosterone were provided by TCI Shanghai (Shanghai, China). Chlorzoxazone and omeprazole were purchased from J&K Chemical (Beijing, China). Heparin sodium injection was provided by the Jiangsu Wanbang Biochemical Pharmaceutical Group Co., Ltd. (Xuzhou, China). Human and rat liver microsomes were purchased from BD Gentest (Franklin, USA). Tris base and MgCl₂ were purchased from Tianjin Yongda Chemical Reagent Co., Ltd. (Tianjin, China). Ultrapure water was prepared by Thermo Scientific Nanopure Water Purifier (Waltham, USA). HPLC-grade methanol and acetonitrile were purchased from Thermo Fisher Scientific (Ottawa, ON, Canada). All the other chemicals were of analytical grade.

DEAE-Sepharose Fast Flow, Superdex 75, and thrombin were purchased from GE Healthcare. Phosphoglucose isomerase from rabbit muscle, phosphomannose isomerase from Escherichia coli, glucose-6-phosphate dehydrogenase from bakers' yeast (Saccharomyces cerevisiae), α-d-glucose 1,6-bisphosphate potassium salt hydrate, α-d-glucose 1-phosphate disodium salt hydrate, and β-nicotinamide adenine dinucleotide phosphate sodium salt were purchased from Sigma-Aldrich. SYPRO Orange was from Invitrogen Molecular Probes. Sodium orthovanadate was from Acros Organics. All other reagents were of analytical grade.

DEAE-Sepharose ff and Superdex-75 were purchased from GE Healthcare Life Sciences, Milan, Italy. Phosphoglucose isomerase from rabbit muscle, phosphomannose isomerase from E. coli, glucose 6-phosphate dehydrogenase from baker’s yeast (S. cerevisiae), phosphoglucomutase from rabbit muscle, α-d(+)Mannose 1-phosphate sodium salt hydrate, and β-Nicotinamide adenine dinucleotide phosphate sodium salt, were purchased from Sigma-Aldrich, Milan, Italy. Sypro Orange was from Invitrogen Molecular Probes, Monza, Italy. Mannose-1,6-bisphosphate was synthesized and purified, as described [3 (link)]. All of the other reagents were of analytical grade.