The total cellular protein was extracted and 25 μg was used for SDS-PAGE. After blocking the membrane for 30 min, rinsing three times with TBST buffer and incubating with anti-NLRP3 (1:800; Abcam) for 12 h at 4°C, the membranes were washed three times in wash buffer (0.2% gelatin, 0.05% Tween 20 in TBS). The membranes were then incubated for 1 h with a horseradish peroxidase-labeled secondary antibody, washed again, and exposed to electrochemiluminescence (ECL) color development reagents. The membranes were developed using the ChemiDoc-It TM TS2 Imaging System (Bio-Rad), and relative optical density was analyzed using ImageJ2x software (National Institute of Health, Bethesda, MD, USA).
Chemidoc it ts2 imaging system
Manufactured by Bio-Rad
Sourced in United States
The ChemiDoc-It TM TS2 Imaging System is a lab equipment product designed for imaging and analyzing biological samples. It provides high-quality image capture and analysis capabilities for various applications in life science research.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using chemidoc it ts2 imaging system
1
NLRP3 Inflammasome Protein Expression
2
Protein Expression Analysis by Western Blot
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Total cellular protein was extracted and 25 μg was used for SDS-PAGE. After blocking the membrane for 30 min, rinsing with TBST buffer three times, and incubation with anti-MAP2 (1:1000; Abcam; ab5392), anti-PKA (1:400; Abcam ab211265), and anti-PPAR-γ (1:500; Abcam ab45036) for 12 h at 4 °C, the membranes were washed three times in wash buffer (0.2% gelatin, 0.05% Tween 20 in TBS). The membranes were then incubated for 1 h with horseradish peroxidase-labeled secondary antibody, washed again, and exposed to ECL color development reagents. The membranes were developed using the ChemiDoc-It TM TS2 Imaging System (Bio-Rad), and relative optical density was analyzed using the ImageJ2x software (National Institute of Health, Bethesda, MD, USA).
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