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Hk080 9k

Manufactured by Merck Group
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The HK080–9K is a laboratory equipment product from Merck Group. It is a high-performance centrifuge designed for a wide range of applications in scientific research and clinical settings. The core function of this equipment is to separate and isolate different components from complex liquid samples through centrifugal force.

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Lab products found in correlation

Vectastain abc hrp kit, by Vector Laboratories (2 mentions) Mouse anti human lag3 igg, by Novus Biologicals (2 mentions) Rabbit anti human tim 3 igg, by Cell Signaling Technology (2 mentions) Biotinylated anti rat igm secondary antibody, by BioLegend (2 mentions) Immpress dab peroxidase substrate, by Vector Laboratories (2 mentions) Nanozoomer slide scanner, by Hamamatsu Photonics (2 mentions)

2 protocols using hk080 9k

1

Immunohistochemical Analysis of Immune Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following heat-induced antigen retrieval for 30 minutes at 95°C or 30 seconds at 125°C in citrate (BioGenex, HK080–9K) or EDTA buffer (Sigma‒Aldrich E1161), slides were incubated with rat anti-mouse/human PNAd IgM (BioLegend, clone MECA-79) at a 1:100 dilution for 1 hour at room temperature, mouse anti-human LAG3 IgG (Novus Biologicals, clone 17B4) at a 1:200 dilution overnight at 4°C, or rabbit anti-human TIM-3 IgG (Cell Signaling Technology, clone D5DR5) at a 1:50 dilution overnight at 4°C, as previously described (Avram et al., 2013 (link); Gorris et al., 2018 (link); Wojcik et al., 2022 (link)). After the washing steps, the slides were incubated with either biotinylated anti-rat IgM secondary antibody (BioLegend, 408903) at a 1:200 dilution followed by the VECTASTAIN ABC-HRP kit (Vector Laboratories, PK-4000), HRP anti-mouse (Vector Laboratories, MP-7452) or HRP anti-rabbit (Vector Laboratories, MP-7451) polymer detection kits. The ImmPRESS DAB peroxidase substrate (Vector Laboratories, SK-4105) was used prior to counterstaining in hematoxylin, dehydration, and mounting with coverslips. Digital images were obtained at 20X magnification via whole-slide brightfield scanning using the NanoZoomer slide scanner (Hamamatsu).
Li K., Tandurella J.A., Gai J., Zhu Q., Lim S.J., Thomas DL I.I., Xia T., Mo G., Mitchell J.T., Montagne J., Lyman M., Danilova L.V., Zimmerman J.W., Kinny-Köster B., Zhang T., Chen L., Blair A.B., Heumann T., Parkinson R., Durham J.N., Narang A.K., Anders R.A., Wolfgang C.L., Laheru D.A., He J., Osipov A., Thompson E.D., Wang H., Fertig E.J., Jaffee E.M, & Zheng L. (2022). Multi-omic analyses of changes in the tumor microenvironment of pancreatic adenocarcinoma following neoadjuvant treatment with anti-PD-1 therapy. Cancer cell, 40(11), 1374-1391.e7.
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2

Immunohistochemical Analysis of Immune Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following heat-induced antigen retrieval for 30 minutes at 95°C or 30 seconds at 125°C in citrate (BioGenex, HK080–9K) or EDTA buffer (Sigma‒Aldrich E1161), slides were incubated with rat anti-mouse/human PNAd IgM (BioLegend, clone MECA-79) at a 1:100 dilution for 1 hour at room temperature, mouse anti-human LAG3 IgG (Novus Biologicals, clone 17B4) at a 1:200 dilution overnight at 4°C, or rabbit anti-human TIM-3 IgG (Cell Signaling Technology, clone D5DR5) at a 1:50 dilution overnight at 4°C, as previously described (Avram et al., 2013 (link); Gorris et al., 2018 (link); Wojcik et al., 2022 (link)). After the washing steps, the slides were incubated with either biotinylated anti-rat IgM secondary antibody (BioLegend, 408903) at a 1:200 dilution followed by the VECTASTAIN ABC-HRP kit (Vector Laboratories, PK-4000), HRP anti-mouse (Vector Laboratories, MP-7452) or HRP anti-rabbit (Vector Laboratories, MP-7451) polymer detection kits. The ImmPRESS DAB peroxidase substrate (Vector Laboratories, SK-4105) was used prior to counterstaining in hematoxylin, dehydration, and mounting with coverslips. Digital images were obtained at 20X magnification via whole-slide brightfield scanning using the NanoZoomer slide scanner (Hamamatsu).
Li K., Tandurella J.A., Gai J., Zhu Q., Lim S.J., Thomas DL I.I., Xia T., Mo G., Mitchell J.T., Montagne J., Lyman M., Danilova L.V., Zimmerman J.W., Kinny-Köster B., Zhang T., Chen L., Blair A.B., Heumann T., Parkinson R., Durham J.N., Narang A.K., Anders R.A., Wolfgang C.L., Laheru D.A., He J., Osipov A., Thompson E.D., Wang H., Fertig E.J., Jaffee E.M, & Zheng L. (2022). Multi-omic analyses of changes in the tumor microenvironment of pancreatic adenocarcinoma following neoadjuvant treatment with anti-PD-1 therapy. Cancer cell, 40(11), 1374-1391.e7.
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