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Silencer select pre designed and validated sirna

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Silencer Select Pre-designed and Validated siRNA is a laboratory product designed to enable gene silencing. It provides pre-designed and validated small interfering RNA (siRNA) sequences targeted to specific gene transcripts. The product is intended to facilitate the study of gene function through RNA interference (RNAi).

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Opti mem 1 reduced serum medium without serum, by Thermo Fisher Scientific (2 mentions) 24 well matrigel coated invasion chambers with an 8 mm pore size, by BD (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Silencer Select Pre-Designed & Validated siRNA Silencer Select Pre-designed siRNA Silencer® Select Validated siRNA Silencer Pre-designed siRNA Validated select siRNA Pre-validated siRNA Pre-designed siRNA Silencer Select siRNAs Silencer Select Silencer siRNA

6 protocols using silencer select pre designed and validated sirna

1

Regulation of HHEX by Vitamin D and Zinc

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Transient transfections of U937-PR9 cells with siRNA were carried out using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Chemically synthesized siRNAs (Silencer Select Pre-designed and Validated siRNA) to HHEX, and scrambled siRNAs were purchased from Ambion and transfected at 10 nM final concentration. After 24 h, the cells were treated with no additives (control) or 50 ng/ml VitD3 or 1 μM Zn2+ or both drugs at the above doses. After 72 h, the expression of HHEX was assayed by Western blot. Cell growth was analyzed using Cell Titer-GloMax assay (Promega, Fitchburg, WI, USA).
Saulle E., Petronelli A., Pelosi E., Coppotelli E., Pasquini L., Ilari R., Lo-Coco F, & Testa U. (2016). PML-RAR alpha induces the downmodulation of HHEX: a key event responsible for the induction of an angiogenetic response. Journal of Hematology & Oncology, 9, 33.
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2

Modulating TRAIL-R2 Expression in Glioma Cells

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Transient transfections of T98G and U251 cells with small interfering (si)RNA were carried out using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Two chemically synthesized siRNAs (Silencer Select Pre-designed and Validated siRNA) to TRAIL-R1 (S16764) and TRAIL-R2 (S16756), and scrambled siRNAs were purchased from Ambion and transfected at 10 nM final concentration. After 72 hours, the cells were treated with no additives (Control) or 50 ng/ml TRAIL or 10 µM Salinomycin or both drugs at the above doses. After 72 hours, the expression of TRAIL-R2 was assayed by flow cytometry.
Calzolari A., Saulle E., De Angelis M.L., Pasquini L., Boe A., Pelacchi F., Ricci-Vitiani L., Baiocchi M, & Testa U. (2014). Salinomycin Potentiates the Cytotoxic Effects of TRAIL on Glioblastoma Cell Lines. PLoS ONE, 9(4), e94438.
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3

siRNA Silencing of HBx and Dll4 Genes

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The siRNA sequences specific for HBx and Dll4 and scrambled siRNA were designed and purchased from Silencer® Select Pre-Designed and Validated siRNA (Life Technologies, USA). Stealth si-HBx RNA sequences have three duplexes as follows: siHBx-199 5’-AGG TGA AGC GAA GTG CAC ATT-3’ [16 (link)], siHBx-260 5’-GAA TGT TGC CCA AGG TCT TAC ATA A-3’ and siHBx-371 5’-GGG AGG AGA TTA GAT TAA AGG TCTT-3’. Stealth si-Dll4 RNA sequences have three duplexes as follows: HSS182569 5’-CCT CTC CAA CTG CCC TTC AAT TTCA-3’, HSS123069 5’-GCC TAT CTG TCT TTC GGG CTG TCAT-3’ and HSS123063 5’-ACC TCC ATT TGT GAT TAG ACA TGTT-3’. Transfection of siRNA was carried out using Lipofectamine RNAiMax Reagent (Life Technologies, USA). After 24–72 hr of transfection, cells were collected for further analysis.
Kongkavitoon P., Tangkijvanich P., Hirankarn N, & Palaga T. (2016). Hepatitis B Virus HBx Activates Notch Signaling via Delta-Like 4/Notch1 in Hepatocellular Carcinoma. PLoS ONE, 11(1), e0146696.
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4

JUN Knockdown in BT-549 Cells

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Knockdown of JUN expression in BT-549 cells was achieved using Silencer select pre-designed and validated siRNA (Life Technologies). Because endogenous c-Jun levels are so high in this cell line, two rounds of siRNA treatment were required to achieve effective knockdowns. Two siRNA products (manufacturer ID # s7658 and s7659) were used, along with the Silencer select negative control no. 1 siRNA. Transfections were carried out in 10 cm dishes at ∼80% confluency using Lipofectamine 2000 transfection reagent (Life Technologies) and following the manufacturer's instructions. The final siRNA concentration was 15 nM in all cases. Transfection medium was replaced with fresh growth medium 5 h after treatment. At 48 h following the initial transfection, the procedure was repeated, and cells were collected after a further 48 h.
Lukey M.J., Greene K.S., Erickson J.W., Wilson K.F, & Cerione R.A. (2016). The oncogenic transcription factor c-Jun regulates glutaminase expression and sensitizes cells to glutaminase-targeted therapy. Nature Communications, 7, 11321.
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5

Evaluating Cell Invasion in Prostate Cells

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For gene-silencing experiments, BPH-1 and RWPE-1 immortalized human prostate cells, obtained from the American Type Culture Collection (ATCC) and previously checked to exclude mycoplasma contamination, were transfected with 10 nM final concentration of siRNA oligonucleotides purchased from Life Technologies (Silencer Select Pre-Designed and Validated siRNAs) using Lipofectamine RNAi Max (Life Technologies) in Opti-MEM I Reduced Serum Medium without serum (Gibco) following the manufacturer’s instructions. Two days later, their invasive potential was evaluated using 24-well Matrigel-coated invasion chambers with an 8 mm pore size (BD Biosciences). For BPH-1, 6 x 105 cells were allowed to invade for 72 h using 15% fetal bovine serum as a chemoattractant, whereas for RWPE-1 cells, 8 x 105 cells were seeded and allowed to invade for 48 h, using bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF) as chemoattractants. Cells that reached the lower surface were stained with crystal violet and counted under the microscope.
de la Rosa J., Weber J., Friedrich M.J., Li Y., Rad L., Ponstingl H., Liang Q., de Quirós S.B., Noorani I., Metzakopian E., Strong A., Li M.A., Astudillo A., Fernández-García M.T., Fernández-García M.S., Hoffman G.J., Fuente R., Vassiliou G.S., Rad R., López-Otín C., Bradley A, & Cadiñanos J. (2017). A single-copy Sleeping Beauty transposon mutagenesis screen identifies new PTEN-cooperating tumor suppressor genes. Nature genetics, 49(5), 730-741.
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6

Evaluating Cell Invasion in Prostate Cells

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For gene-silencing experiments, BPH-1 and RWPE-1 immortalized human prostate cells, obtained from the American Type Culture Collection (ATCC) and previously checked to exclude mycoplasma contamination, were transfected with 10 nM final concentration of siRNA oligonucleotides purchased from Life Technologies (Silencer Select Pre-Designed and Validated siRNAs) using Lipofectamine RNAi Max (Life Technologies) in Opti-MEM I Reduced Serum Medium without serum (Gibco) following the manufacturer’s instructions. Two days later, their invasive potential was evaluated using 24-well Matrigel-coated invasion chambers with an 8 mm pore size (BD Biosciences). For BPH-1, 6 x 105 cells were allowed to invade for 72 h using 15% fetal bovine serum as a chemoattractant, whereas for RWPE-1 cells, 8 x 105 cells were seeded and allowed to invade for 48 h, using bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF) as chemoattractants. Cells that reached the lower surface were stained with crystal violet and counted under the microscope.
de la Rosa J., Weber J., Friedrich M.J., Li Y., Rad L., Ponstingl H., Liang Q., de Quirós S.B., Noorani I., Metzakopian E., Strong A., Li M.A., Astudillo A., Fernández-García M.T., Fernández-García M.S., Hoffman G.J., Fuente R., Vassiliou G.S., Rad R., López-Otín C., Bradley A, & Cadiñanos J. (2017). A single-copy Sleeping Beauty transposon mutagenesis screen identifies new PTEN-cooperating tumor suppressor genes. Nature genetics, 49(5), 730-741.
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