Legend max human il 6 elisa kit

SIL-6, ESR, and CRP levels were measured upon admission (within 24 h before TKA procedure) and at 24 h, 72 h, and 4 weeks after surgery. JIL-6 levels were obtained during the TKA procedure by aspiration of fluid from the knee joint before arthrotomy, at joint space closure and at 24 h after surgery via clamped suction tube drainage.
All samples were immediately placed on ice for transfer. Samples were centrifuged at 1000g for 15 min to remove cells and serum samples were stored at −80 °C until analysis. IL-6 concentration was determined by human enzyme-linked immunosorbent assay (LEGEND MAX™ Human IL-6 ELISA Kit; BioLegend, Inc., San Diego, CA, USA) using standard concentration-optical density curve. ESR level was analyzed using a Monitor-20 ESR analyzer (Vital Diagnostics, Lincoln, RI, USA) and CRP level was determined using BN ProSpec^® System (Siemens Healthcare GmbH, Erlangen, Germany).

Secreted IL-6, IL-11, LIF, and OSM in the media supernatant of cultured cells was quantified using a LEGEND MAX Human IL-6 ELISA Kit (Biolegend #430507), Human LIF ELISA Kit (Raybiotech #ELH-LIF-1, Peachtree Corners, GA, USA), Human OSM ELISA Kit (Raybiotech #ELH-OSM-1), and Human IL-11 ELISA Kit (Raybiotech #ELH-IL11-1), as per the manufacturer’s instructions. Absorbance at 450 nm was measured on a microplate reader (PE Enspire).

IL-6 cytokine secreted by cells was quantified using LEGEND MAX Human IL-6 ELISA Kit (Biolegend #430507). Media supernatant collected from culture of various cells were incubated and stained with reagents on a pre-coated 96-well strip plate as per manufacturer’s instructions. A microplate reader (Enspire) was used to measure the absorbance at 450 nm.
Quantibody^® Human Cytokine Array 1 (RayBiotech) is a multiplex ELISA system for quantitative measurement of multiple cytokines simultaneously. Sample preparation and analysis was performed according to the manufacturer’s instructions. Further statistical analysis was performed using GraphPad.