Mcc950 sodium

MCC950 (sodium) (molecular weight = 426.46, molecular formula: C₂₀H₂₃N₂NaO₅S, CAS NO: 256373-96-3, purity > 99.40%) was obtained from MedChem Express (Shanghai, China). Normal saline was produced from Shandong Hualu Pharmaceutical Co., Ltd. (Liaocheng, China). Insulin injection was supplied from WanbangBioPharma (Xuzhou, China). The ELISA kit for measuring IL-1β, the caspase-1 activity assay kit, and the Bradford protein assay kit were purchased from Beyotime Institute of Biotechnology (Beijing, China). The ELISA kit for determining the mouse insulin level was obtained from ALPCO (Salem, MA, USA). The ELISA kit for measuring TNF-α was purchased from DAKEWEI (Shenzhen, China). Primary antibody against NLRP3 (ab214185) was obtained from Abcam (Cambridge, UK). Primary antibodies against ASC (sc-514414) and IL-1β (sc-7884) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The peroxidase-conjugated secondary antibodies of goat anti-rabbit IgG (ZB-2301) and goat anti-mouse IgG (ZB-2305) were purchased from ZSJQ-BIO (Beijing, China). Primary antibody against β-actin (CW0096) and kits for tissue protein extraction, protease inhibitor, bicinchoninic acid (BCA) protein quantization, and enhanced chemiluminescence regeat were bought from CoWin Biosciences (Beijing, China).

MCC950 sodium and dihydroethidium (DHE) were purchased from MedChemExpress (MCE R; China). (3,4-dihydroxyphenethyl) methacrylamide (DPMA) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Poly (ethylene glycol) methacrylate (PEGMA) was purchased from Aladdin Reagent Co., Ltd. and passed through an Al₂O₃ column to remove the inhibitor before use. 3-acrylamidopropyl trimethylammonium chloride (APTAC, 75wt% in H₂O) and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, 98%) were obtained from Aladdin Reagent Co., Ltd and used without further purification. Fluorescein sodium (FS) was purchased from Abmole Co., Ltd. (United States). Scopolamine (Scop) was purchased from Sigma–Aldrich Co., Ltd. Human corneal epithelial cells (HCECs) were obtained from ATCC (Manassas, VA, United States). Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 (DMEM/F-12; 1: 1 ratio), FBS, and insulin were purchased from Invitrogen, Gibco Co., Ltd. Triton X-100 was purchased from Sigma Co., Ltd. (Darmstadt, Germany). CM-H2DCFDA (General Oxidative Stress Indicator) was obtained from Invitrogen Molecular Probe Co., Ltd. Cell Counting Kit-8 (CCK-8) was purchased from Beyotime Biotechnology Co. (Shanghai, China). The In situ Cell Death Detection kit (TUNEL) was purchased from Roche Co., Ltd. (Germany).