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2 protocols using mouse anti arginase 1

1

Neuroprotective Mechanism of PEG-PCL Micelles

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Rosuvastatin was obtained from Solarbio (IR0150, Solarbio, Beijing, China). Poly (ethylene glycol) 2000-block-poly(ε-caprolactone) 3000 (PEG-PCL) was purchased from Shanghai ToYongBio Tech. Inc. (Shanghai, China). Fluoro-Jade C dyestuff was obtained from Merck (AG325-30MG, Millipore, Darmstadt, Germany). Rabbit anti-iNOS, rabbit anti-β-Tublin and mouse anti-Arginase 1 were purchased from Proteintech (1895-1-AP, 10094-1-AP, 66129-1-Ig, Proteintech, Wuhan, China). Goat anti-Iba-1 were purchased from Wako Pure Chemical Industries (011-27991, Wako, Osaka, Japan). Rabbit anti-IL-1β was supplied from Abbkine (ABP52932, Abbkine, Redlands, California). Rabbit anti- TNF-α was purchased from HuaAn Biotechnology (ER1919-22, HuaAnBio, Hangzhou, China). Rabbit anti- IL-10 was supplied from Wanleibio (WL03088, Wanleibio, Shenyang, China) Fluorescent Alexa 488 and 555 were purchased from Invitrogen (A-21121, A-11008, A-21432, Invitrogen, CA, USA). Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L), AffiniPure Goat Anti-Rabbit IgG (H+L) were purchased from Jackson (115-035–003, 111-005-003, Jackson ImmunoResearch, PA, USA). The secondary antibodies for WB were goat anti-mouse IgG-HRP and goat anti-rabbit IgG-HRP (ZB-2301; ZB-2305; Zhongshan Golden Bridge, Beijing, China).
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2

Hydrogel-Based In Vitro Model

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Gelatin (type B ∼ 225 bloom), N-(3-Dimethylaminopropyl)-N’-ethyl carbodiimide hydrochloride (EDAC), 5, 5′-dithiobis (2-nitrobenzoic acid) (DTNB), polyethylene glycol diacrylate (PEGDA, MW 6000 Da), cysteamine, and dithiothreitol (DTT) were purchased from Sigma (St. Louis, MO, United States). N-hydroxysuccinimide (NHS) was obtained from Pierce. Viability/Cytotoxicity Assay Kit was purchased from Invitrogen. Primary antibodies consisted of mouse anti-GFAP, rabbit anti-Iba-1, rabbit anti-iNOS, mouse anti-Arginase 1, and rabbit anti-neun were purchased from Proteintech (United States). Rabbit anti-integrin β1 was purchased from Huaan (China). Rabbit anti-IL-1β and TNF-α were obtained from Santa Cruz Biotechnology (United States). Secondary antibodies consisted of fluorescent Alexa 488 and 555 antibodies were purchased from Invitrogen (United Kingdom). Horse radish peroxidase (HRP-conjugated AffiniPure goat anti-rabbit) was obtained from Jackson (United States).
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