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Tnf α m elisa kit

Manufactured by Boster Bio
Sourced in China

The TNF-α (m) ELISA kit is a laboratory instrument used for the quantitative measurement of mouse tumor necrosis factor-alpha (TNF-α) levels in biological samples. It is a sensitive and specific enzyme-linked immunosorbent assay (ELISA) designed to detect and quantify TNF-α concentrations.

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3 protocols using tnf α m elisa kit

1

Serum Inflammatory Markers Quantification

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Serum levels of IL-1β (IL-1β (m) ELISA kit, EK0394, Boster), IL-18 (IL-18 (m) ELISA kit, #EMC011, NeoBioscience), IL-6 (IL-6 (m) ELISA kit, EK0411, Boster), TNF-α (TNF-α (m) ELISA kit, EK0527, Boster) and NO production (Nitrate/Nitrite Assay Kit, S0023, Beyotime, China) were evaluated using commercially available kits according to the manufacturer's instructions.
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2

Cytokine and Nitric Oxide Evaluation

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Levels of IL-1β (IL-1β (m) ELISA kit, EK0394, Boster), IL-18 (IL-18 (m) ELISA kit, #EMC011, NeoBioscience), IL-6 (IL-6 (m) ELISA kit, EK0411, Boster), TNF-α (TNF-α (m) ELISA kit, EK0527, Boster) and NO production (Nitrate/Nitrite Assay Kit, S0023, Beyotime, China) were evaluated using commercially available kits according to the manufacturer's instructions.
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3

Cytokine Detection in BV2 Cells

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Cytokines were detected using an enzyme-linked immunosorbent assay (Kim and Weiser, 1998 (link)) as reported earlier with some modifications (Segura et al., 1999 ; Dominguez-Punaro et al., 2010 (link)). The SS2 wild-type strain ZY05719, mutant and complementation strains were added to BV2 at an MOI of 2 for 12 h at 37°C under 5% CO2. Commercial ELISA kits (Boster, TNF-α (m) ELISA kit, Cat.No.EK0525, IFN-γ (m) ELISA kit, Cat.No.EK0375) were used to measure the production of TNF-α and IFN-γ in the harvested culture supernatant.
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