Xanthohumol xn

Xanthohumol (XN), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). XN was dissolved in DMSO into stock concentrations of 50mM. Antibodies against survivin, glyceraldehyde phosphate dehydrogenase (GAPDH), Notch1 (epitope from C-terminus), HES-1, cyclin D1, Bcl2, Mcl1, total PARP, and caspase-3 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and cleaved caspase-3 and cleaved PARP were obtained from Cell Signaling Technology Inc. (Danvers, MA, USA) respectively.

Xanthohumol (Xn) and 7-ethyl-10-hydroxycamptothecin (SN38) were purchased from Sigma-Aldrich (St. Quentin Fallavier, France) and prepared in dimethyl sulfoxide (DMSO). Anti-Cyclin A antibody (sc-751; 1:1000), Cyclin B (sc-752; 1:1000), Cyclin E (sc-481; 1:1000), Cdk1 (sc-54; 1:1000), Cdk2 (sc-6248; 1:500), γH2AX (Ser¹³⁹) (sc-101696; 1:500), and p21 (sc-756; 1:500) were obtained from Santa Cruz (Nanterre, France). Anti-ATM antibody (#2873; 1:1000), p-ATR (Ser⁴²⁸) (#2853; 1:1000), and p-p53 (Ser¹⁵) (#9284; 1:1000) were purchased from Cell Signaling (Ozyme, Saint-Cyr-l’École, France). Anti-p-ATM (Ser¹⁹⁸¹) antibody (ab81292; 1:10000) and p53 (ab131442; 1:500) were obtained from Abcam (Paris, France). Anti-β-actin antibody (#A1978; 1:2000) was obtained from Sigma-Aldrich (St. Quentin Fallavier, France).