To characterize the redox potential of pulcherrimin, cyclic voltammetry was performed to evaluate to what extent pulcherrimin immobilizes Fe(III) compared to known Fe chelators such as deferoxamine and EDTA. Fe-Deferoxamine et Fe-EDTA complexes were pre-formed for 1 h in the dark. For Fe-PA, the complexation was performed in the electrochemical cell 3 min prior to collecting the first voltammogram. The experiment was conducted on a CHI-1040C (CH Instruments). A 3 mm diameter glassy carbon working electrode (CHI104), a platinum plate (counter electrode), and an Ag/AgCl (KCl saturated; reference electrode) were used and acquired from CH Instruments. All voltammetric experiments were performed in deoxygenated PBS with a constant flow of argon by cycling the potential between −1.0 and 1.0 V at a scan rate of 25 mV·s−1. All solutions were evaluated with 10−4 M of Fe (ratio 1:1; Ligand-Metal) except for pulcherriminic acid, where 2 × 10−4 M was added (3:2 ratio; PA-Fe).
Ag agcl
Manufactured by CH Instruments
Sourced in United States
The Ag/AgCl (silver/silver chloride) electrode is a type of reference electrode commonly used in electrochemical measurements. It consists of a silver wire coated with silver chloride, immersed in a solution containing chloride ions. The Ag/AgCl electrode provides a stable and reproducible reference potential, which is essential for accurate electrochemical measurements.
Automatically generated - may contain errors
4 protocols using ag agcl
1
Electrochemical Characterization of Pulcherrimin
2
Electrochemical Characterization Setup
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All electrochemistry
experiments were performed with a CH Instrument (CHI660E) potentiostat.
A 3-electrode setup was used for all experiments. Working electrodes
were R2 mm GC discs (CH Instruments). Reference electrodes were Ag/AgCl
(+0.197 V vs SHE) (CH Instruments). Counter electrodes were platinum-coated
titanium rods (EDAQ Inc.).
experiments were performed with a CH Instrument (CHI660E) potentiostat.
A 3-electrode setup was used for all experiments. Working electrodes
were R2 mm GC discs (CH Instruments). Reference electrodes were Ag/AgCl
(+0.197 V vs SHE) (CH Instruments). Counter electrodes were platinum-coated
titanium rods (EDAQ Inc.).
3
Photoexcitation of FTO-Supported Catalysts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluorine-doped tin oxide (FTO) (1.5 × 2 cm2) was used as a substrate to support samples (10 mg) that were ultrasonically dispersed in 20 mL ethanol and then added dropwise to coat the FTO. The sample loaded FTO was then dried at 60 °C for 2 h and a 1.5 × 1.5 cm2 sample area was used for the photoexcitation experiment.
The photocurrent was measured on a CHI 660E electrochemical workstation with a three-electrode system, in which the prepared electrode was used as the working electrode, a Pt wire (CH Instruments, Inc.) was used as the counter electrode, and a Ag/AgCl (CH Instruments, Inc.) electrode was used as the reference electrode. A 0.5 M Na2SO4 solution was used as the electrolyte. A Xe lamp (CEL-HXF300, CEAULIGHT) with AM 1.5 filter (CEL-AM 1.5, CEAULIGHT) was used as the light source. The light intensity was regulated to 50 mW·cm−2 by adjusting the distance between the light source and the sample.
The photocurrent was measured on a CHI 660E electrochemical workstation with a three-electrode system, in which the prepared electrode was used as the working electrode, a Pt wire (CH Instruments, Inc.) was used as the counter electrode, and a Ag/AgCl (CH Instruments, Inc.) electrode was used as the reference electrode. A 0.5 M Na2SO4 solution was used as the electrolyte. A Xe lamp (CEL-HXF300, CEAULIGHT) with AM 1.5 filter (CEL-AM 1.5, CEAULIGHT) was used as the light source. The light intensity was regulated to 50 mW·cm−2 by adjusting the distance between the light source and the sample.
4
Au Nanostructures Electrodeposition Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
As substrates for electrodeposition, Au disk electrodes (Bioanalytical Systems, Inc., West Lafayette, IN, USA, 2 mm in diameter) were used. An Ag/AgCl (CH Instruments, Inc., Austin, TX, USA) and an Au wire (Sigma-Aldrich, St. Louis, MO, USA, 1 mm in diameter) was used as the reference and counter electrode, respectively.
Before the electrodeposition of Au nanostructures, Au disk substrate electrodes were wet-polished on a polishing cloth with 0.3-µm alumina powder. To remove the alumina slurry, the electrodes were sonicated in deionized water for 10 min. A deposition solution was an aqueous solution containing 7 mM HAuCl4 and 0.1 mM Pb(CH3COO)2 that was purged using Ar gas for 10 min before deposition. Au nanostructured layers were electrodeposited on the Au disk electrodes in the prepared solution with amperometry up to the deposition charge of 0.04 C at various applied potentials: 0.05, 0.15, 0.20, 0.25 and 0.45 V (vs. Ag/AgCl). A CHI 900B bipotentiostat (CH Instruments, Inc., Austin, TX, USA) was used for electrodeposition.
Before the electrodeposition of Au nanostructures, Au disk substrate electrodes were wet-polished on a polishing cloth with 0.3-µm alumina powder. To remove the alumina slurry, the electrodes were sonicated in deionized water for 10 min. A deposition solution was an aqueous solution containing 7 mM HAuCl4 and 0.1 mM Pb(CH3COO)2 that was purged using Ar gas for 10 min before deposition. Au nanostructured layers were electrodeposited on the Au disk electrodes in the prepared solution with amperometry up to the deposition charge of 0.04 C at various applied potentials: 0.05, 0.15, 0.20, 0.25 and 0.45 V (vs. Ag/AgCl). A CHI 900B bipotentiostat (CH Instruments, Inc., Austin, TX, USA) was used for electrodeposition.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!