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2 protocols using poly ethylene glycol peg mw 07435

1

Fabrication of Microfluidic Devices

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Deionized water was further purified to 18 MΩ using a NANOpure II Water System (“Nanopure water”; Barnstead Thermolyne Corporation, Ramsey, MN). Polydimethylsiloxane (PDMS) was made from 184 Silicone Elastomer Base and 184 Silicone Elastomer Curing Agent (Dow Chemical Company, Midland, MI) in a 7:1 ratio and cured under vacuum at 50 °C. Sodium chloride, magnesium chloride hexahydrate, RNaseZap, UltaPure agarose, SyberSafe DNA gel stain, and SyberGold DNA gel stain were purchased from Thermofisher Scientific (Waltham, MA). Sodium citrate was purchased from Mallinckrodt (Staines-upon-Thames, UK), and Tween 20 and 10x TBE buffer from Bio-Rad (Hercules, CA). Poly(ethylene glycol) (PEG) (MW 07435) was purchased from Sigma-Aldrich (Munich, Germany). All 3D printed structures were designed using SolidWorks 2020 Student Edition and printed using 1.75 mm PLA Filament (Hatchbox 3D, Pomona, CA) on an Original Prusa i3 MK3S+ 3-D Printer (Prusa Research, Prague, Czech Republic). All statistical data was analyzed using JMP Pro 16.
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2

Fabrication of Microfluidic Devices

Check if the same lab product or an alternative is used in the 5 most similar protocols
Deionized water was further purified to 18 MΩ using a NANOpure II Water System (“Nanopure water”; Barnstead Thermolyne Corporation, Ramsey, MN). Polydimethylsiloxane (PDMS) was made from 184 Silicone Elastomer Base and 184 Silicone Elastomer Curing Agent (Dow Chemical Company, Midland, MI) in a 7:1 ratio and cured under vacuum at 50 °C. Sodium chloride, magnesium chloride hexahydrate, RNaseZap, UltaPure agarose, SyberSafe DNA gel stain, and SyberGold DNA gel stain were purchased from Thermofisher Scientific (Waltham, MA). Sodium citrate was purchased from Mallinckrodt (Staines-upon-Thames, UK), and Tween 20 and 10x TBE buffer from Bio-Rad (Hercules, CA). Poly(ethylene glycol) (PEG) (MW 07435) was purchased from Sigma-Aldrich (Munich, Germany). All 3D printed structures were designed using SolidWorks 2020 Student Edition and printed using 1.75 mm PLA Filament (Hatchbox 3D, Pomona, CA) on an Original Prusa i3 MK3S+ 3-D Printer (Prusa Research, Prague, Czech Republic). All statistical data was analyzed using JMP Pro 16.
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