Xrcc5

Manufactured by Santa Cruz Biotechnology

Sourced in United States

XRCC5 is a protein that plays a crucial role in the non-homologous end joining (NHEJ) pathway, which is a DNA repair mechanism. XRCC5 is involved in the recognition and binding of DNA double-strand breaks, facilitating the repair process. This product is intended for use in research applications related to DNA repair and genomic stability.

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Lab products found in correlation

Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions) γh2ax, by Merck Group (1 mentions) Ar v7, by Abcam (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Bio-Rad (1 mentions) Yh2ax, by Cell Signaling Technology (1 mentions) Dna pkcs, by Novus Biologicals (1 mentions)

Spelling variants of this product

Rabbit anti-XRCC5 (2 citations) Anti-XRCC5 (2 citations)

2 protocols using xrcc5

Western Blot Analysis of DNA Repair Factors

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For western blot analyses, protein extracts of each sample (50 µg/lane) were electrophoretically separated and transferred onto PVDF membranes. After blocking membranes with 5% non-fat milk TBST solution, they were incubated with appropriate dilutions of specific primary antibodies against ARv7 (#ab198394, abcam, USA), AR (#sc-816, Santa Cruz, USA), γ-H2AX (#05–636 clone JBW301, EMD Millipore), XRCC5 (#sc-5309, Santa Cruz, USA), SPRTN (#PA5-110,424,Thermofisher, USA) and GAPDH (#sc-166, Santa Cruz, USA), followed by horseradish peroxidase-conjugated secondary antibody. The blots were then incubated with HRP-conjugated secondary antibodies and were detected by SuperSignal™ West Femto Maximum Sensitivity Substrate (#34,095, ThermoFisher Scientific) using the Bio-Rad imaging system.

Chen D., Chou F.J., Chen Y., Huang C.P., Tian H., Wang Y., Niu Y., You B., Yeh S., Xing N, & Chang C. (2022). Targeting the radiation-induced ARv7-mediated circNHS/miR-512-5p/XRCC5 signaling with Quercetin increases prostate cancer radiosensitivity. Journal of Experimental & Clinical Cancer Research : CR, 41, 235.

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Antibody Usage in Western Blotting and ChIP

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We used the following commercial antibodies: JARID2 (NB100-2214; Novus Biologicals, Littleton, CO), CYLD (sc-28211; Santa Cruz Biotechnology, Santa Cruz, CA), XRCC5 (sc-9034; Santa Cruz Biotechnology), XRCC6 (ALS14031; Abgent, San Diego, CA), TBX2 (H00006909-M01; Novus Biologicals), H3 (4499; Cell Signaling, Danvers, MA), DDB2 (Western blotting: 5416; Cell Signaling; ChIP: sc-25368; Santa Cruz Biotechnology), DNA-PKcs (NBP2-22128; Novus Biologicals), SEMA3A (AF1250; Novus Biologicals), actin (A4700; Sigma-Aldrich, St. Louis, MO), and y-H2AX (9718; Cell Signaling). DDB1 and CUL4A antibodies were described previously (Nag et al., 2004 (link)). Horseradish peroxidase–conjugated secondary antibodies were purchased from Bio-Rad.

Fantini D., Huang S., Asara J.M., Bagchi S, & Raychaudhuri P. (2017). Chromatin association of XRCC5/6 in the absence of DNA damage depends on the XPE gene product DDB2. Molecular Biology of the Cell, 28(1), 192-200.

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