Human opn duoset elisa development system kit

To identify FL-OPN, an ELISA kit (JP27158, IBL, Gunma, Japan) was used [3 (link)]. In the FL-OPN kit, O-17, a polyclonal rabbit antibody specific to Iso17-Gln31, was used as a capture antibody, and the mouse monoclonal antibody specific to Lys166–Glu187, 10A16, served as the detector antibody. These combinations of antibodies allowed us to detect FL-OPN [13 (link)]. Ud-OPN in the culture supernatants was detected using a Human OPN DuoSet ELISA Development System Kit (R&D Systems, Minneapolis, MN, USA) [12 (link)]. The proprietary capture monoclonal antibody and the detection of polyclonal antibodies in this ELISA kit were both generated against recombinant human OPN (NS0-derived; amino acids, Ile17-Asn300); the epitopes for these antibodies were not disclosed. To expose the protein in the Exo, samples containing the Exo and control antigens were suspended with PBS containing Triton X-100 (1%) for 30 min at room temperature.

To identify FL-OPN, an ELISA kit (JP27158, IBL, Gunma, Japan) was used. Ud-OPN was determined using the Human OPN DuoSet ELISA Development System kit (DY1433, R&D Systems, Minneapolis, MN, USA) [27 (link)].

To identify FL-OPN, an ELISA kit (JP27158, IBL, Gunma, Japan) was used. Ud-OPN was determined using the Human OPN DuoSet ELISA Development System kit (DY1433, R&D Systems, Minneapolis, MN, USA) [9 (link)].