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Pmirglo reporter gene vector

Manufactured by Promega
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Sourced in United States

The PmirGlo reporter gene vector is a plasmid that contains a firefly luciferase gene under the control of a promoter. This vector can be used to measure gene expression levels in a variety of cell types.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Dual luciferase reporter assay kit, by Promega (2 mentions)

Close spelling variants of this product

PmiRGLO dual-luciferase reporter gene vector PmirGLO luciferase reporter gene vector PmirGLO reporter vector PmirGLO reporter PmirGLO vector Reporter vectors PmirGLO

2 protocols using pmirglo reporter gene vector

1

Validating MAPK1 and GATA3 Regulatory Elements

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The MAPK1 promoter and GATA3 3′-untranslated region (UTR) were cloned into the pmirGlo reporter gene vector (Promega, Madison, WI, United States) and were validated through DNA sequencing. A KOD-Plus Mutagenesis Kit (Toyobo Biochemicals, Osaka, Japan) was used to mutate the presumed binding sites according to the protocols. Lipofectamine 2000 (Invitrogen) was employed for cell transfection in 6-well plates, and 48h later, a dual-luciferase reporter assay kit (Promega) was applied to detect luciferase activity following the manufacturer’s instructions.
Guo Q., Shi D., Lin L., Li H., Wei Y., Li B, & Wu D. (2021). De-Ubiquitinating Enzymes USP21 Regulate MAPK1 Expression by Binding to Transcription Factor GATA3 to Regulate Tumor Growth and Cell Stemness of Gastric Cancer. Frontiers in Cell and Developmental Biology, 9, 641981.
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2

Validation of SNHG16 and USP21 Interactions

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The SNHG16 promoter or the predicted miR-4500 binding sequence in SNHG16 and USP21 3′-untranslated region (UTR) were cloned into the pmirGlo reporter gene vector (Promega, Madison, WI, USA) and were verified by sequencing. The putative binding sites were mutated using a KOD-Plus Mutagenesis Kit (Toyobo Biochemicals, Osaka, Japan) to abolish their binding. Cells were transfected with Lipofectamine 2000 (Invitrogen) in 6-well plates, and luciferase activity was measured 48 h after transfection using a Dual Luciferase Reporter Assay Kit (Promega) according to the manufacturer’s protocol.
Xu P., Xiao H., Yang Q., Hu R., Jiang L., Bi R., Jiang X., Wang L., Mei J., Ding F, & Huang J. (2020). The USP21/YY1/SNHG16 axis contributes to tumor proliferation, migration, and invasion of non-small-cell lung cancer. Experimental & Molecular Medicine, 52(1), 41-55.
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