Superscript 3 reverse transcriptase kit

Total RNAs were extracted using Trizol reagent according to the manufacturer’s specifications (TaKaRa Bio, Dalin, China). One microgram of total RNA from each sample was subjected to cDNA synthesis using the SuperScript III Reverse Transcriptase Kit (TaKaRa Bio, Dalin, China), for detection of the indicated genes and the housekeeping gene ACTIN. Each cDNA sample was amplified using SYBR Green (TaKaRa Bio, Dalin, China). The relative expression of the reported genes was determined using real-time PCR performed using an Applied Biosystems 7900HT Fast Real-Time PCR system. Fold change for every gene was calculated by the 2^−ΔΔCt method. Primers used for real-time PCR were as follows: Actin: F: 5’-TGACGTGGACATCCGCAAAG-3’; R: 5’-CTGGAAGGTGGACAGCGAGG-3′; FKBP10: F: 5’-GGCAGGGTTACATCATCCCC-3’; R: 5’-AAGATTAGCACGGCAGAGCC-3′; FKBP11; F: 5’-GTGTGTGGGAGAGAAGCGAA-3’; R: 5’-TGCAATCAGCTCCACGTCAT-3′.

RNAs were extracted using Trizol (TaKaRa Bio, Dalin, China). One microgram of total RNA was used for cDNA synthesis by the SuperScript III Reverse Transcriptase Kit (TaKaRa Bio, Dalin, China). For detection of the indicated genes, each cDNA sample was amplified using SYBR Green (TaKaRa Bio, Dalin, China) via QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). Primers used for PCR were showed in Supplementary Table 2.