The cells were fixed in 4% paraformaldehyde solution and 1% Triton solution was added to penetrate the cell membrane. Normal sheep serum ((DAKO, Germany) was then used to block the cells at room temperature for 30 minutes. After incubating with primary antibodies (Active-β-Catenin Antibody, Merck, Germany, Cat No: 05-665-25UG ,1:100) at 4°C for 12-16 hours, Primary antibody binding and nucleus were detected in the dark using fluorescence secondary antibody (1:100) and Hoechst staining kit, respectively. A laser scanning confocal microscope (Leica, Germany) was used to observe the cells. In each experiment, at least 200 cells were counted.
Active β catenin antibody
Manufactured by Merck Group
Sourced in Germany
The Active-β-Catenin Antibody is a laboratory tool used to detect and measure the active form of the β-catenin protein. β-catenin is a key component of the Wnt signaling pathway, which plays a crucial role in various cellular processes, such as cell growth, differentiation, and development. The Active-β-Catenin Antibody specifically recognizes the active, non-phosphorylated form of β-catenin, allowing researchers to study the activation of this important signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using active β catenin antibody
1
Immunofluorescence Staining for Active β-Catenin
2
Western Blot Antibody Sources
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