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Tecnai g2 f20 st

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The Tecnai G2 F20 ST is a high-performance transmission electron microscope (TEM) designed for advanced materials characterization. It offers a range of advanced imaging and analytical capabilities to support materials science research and development.

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Jsm 7500f, by JEOL (1 mentions) Ultrascan ccd camera, by Ametek (1 mentions) Fei vitrobot mark 4, by Thermo Fisher Scientific (1 mentions)

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Tecnai G2 F20 (520 citations) Tecnai F20 (449 citations) Tecnai G2 (325 citations) Tecnai G2 F20 ST TEM (4 citations) Tecnai G2 F20 ST Transmission Electron Microscope (2 citations)

3 protocols using tecnai g2 f20 st

1

Structural Analysis of Vanadium Oxide Nanowires

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The particle morphologies of α-V2O5 and ξ-V2O5 nanowires were examined by SEM using a JEOL JSM-7500F instrument operating at an accelerating voltage of 5 kV. TEM images, along with selected-area electron diffraction patterns, were acquired using an FEI Tecnai G2 F20 ST instrument operated at an accelerating voltage of 200 kV. TEM samples were prepared by dispersing into 2-proponal, then drop casting onto 400-mesh grids with holey-carbon films supported by copper (Electron Microscopy Science). In situ heating XRD was performed with a Bruker D8-Vario diffractometer with a Lynxeye detector and MTC oven. The powder sample was mounted on a ceramic heating stage under an N2 atmosphere. Data were collected from 5 to 70° 2θ with a step size of 0.014° and a dwell time of 2 s for ∼1 h scans, with 1-h delays to allow sample equilibration at each temperature. In situ heating XRD measurements of ζ-V2O5 were performed from 30 to 600 °C.
Luo Y., Rezaei S., Santos D.A., Zhang Y., Handy J.V., Carrillo L., Schultz B.J., Gobbato L., Pupucevski M., Wiaderek K., Charalambous H., Yakovenko A., Pharr M., Xu B.X, & Banerjee S. (2022). Cation reordering instead of phase transitions: Origins and implications of contrasting lithiation mechanisms in 1D ζ- and 2D α-V2O5. Proceedings of the National Academy of Sciences of the United States of America, 119(4), e2115072119.
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2

Characterization of Sealant Morphology

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5 µL of sealant solution was drop casted on a freshly glow-discharged carbon coated 300 mesh copper grid and incubated for 5 min at room temperature. The excess solution was wiped out and washed with water and then stained with 2% (w/v) uranyl acetate. The morphology of sealants were characterized once by using Transmission Electron Microscope (Tecnai G2-F20 ST, FEI Company) with accelerated voltage of 120 kV and 200 kV (Figure S9).
Ghosh S., Mukherjee S., Dutta C., Chakraborty K., Gayen P., Jan S., Bhattacharyya D, & Roy R.S. (2017). Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting. Scientific Reports, 7, 6509.
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3

Cryo-TEM Visualization of Extracellular Vesicles

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MM1.R and RPMI-8226 derived extracellular vesicles were prepared for cryo-TEM as described previously [13 (link)]. Briefly, 4 μL of vesicle suspensions were applied to glow discharged lacey carbon coated copper grids (400 mesh, Pacific Grid-Tech, San Francisco, CA) in a controlled environment (22 °C and 95 % relative humidity) using an automated vitrification device (FEI Vitrobot Mark IV, FEI, Hillsboro, OR) and flash-frozen in liquid ethane. Vesicles were visualized in a FEI Tecnai G2 F20 ST transmission electron microscope (TEM, FEI, Hillsboro, OR) operated at 200 kV under low dose radiation. Images were captured on a Gatan Ultrascan CCD camera (38,000× magnification).
Harshman S.W., Canella A., Ciarlariello P.D., Agarwal K., Branson O.E., Rocci A., Cordero H., Phelps M.A., Hade E.M., Dubovsky J.A., Palumbo A., Rosko A., Byrd J.C., Hofmeister C.C., Benson DM J.r., Paulaitis M.E., Freitas M.A, & Pichiorri F. (2016). Proteomic Characterization of Circulating Extracellular Vesicles Identifies Novel Serum Myeloma Associated Markers. Journal of proteomics, 136, 89-98.
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