Thirty-seven component fatty acid methyl esters (FAMEs), thin-layer chromatography (TLC) standards (oleic acid, monoolein, diolein, triolein 1:1:1:1), and Rhizopus oryzae lipase were purchased from Sigma Aldrich (St. Louis, MO, USA). Porcine pancreatic lipase, pepsin, trypsin, and Ox gall salt were purchased from Beijing Solable Technology Co., Ltd. (Beijing, China). All other high-purity reagents were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China).
Rhizopus oryzae lipase
Manufactured by Merck Group
Sourced in United States
Rhizopus oryzae lipase is a microbial enzyme derived from the fungus Rhizopus oryzae. It is an extracellular lipase that catalyzes the hydrolysis of various lipids, including triglycerides, phospholipids, and fatty acid esters. The enzyme exhibits a wide range of substrate specificity and can be used in various industrial and research applications.
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Lab products found in correlation
Triolein, by Merck Group (1 mentions)
Oleic acid, by Merck Group (1 mentions)
Monoolein, by Merck Group (1 mentions)
Diolein, by Merck Group (1 mentions)
Glutaraldehyde ga, by Merck Group (1 mentions)
Coomassie brilliant blue g 250, by Merck Group (1 mentions)
Iron 2 sulfate tetrahydrate, by Merck Group (1 mentions)
3 aminopropyltriethoxysilane, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Orthophosphoric acid, by Merck Group (1 mentions)
Absolute ethanol, by Merck Group (1 mentions)
Iron 3 chloride hexahydrate, by Merck Group (1 mentions)
Gum arabic, by Merck Group (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Esterase, by Merck Group (1 mentions)
Lipase from aspergillus niger, by Merck Group (1 mentions)
Thermomyces lanuginosus lipase, by Merck Group (1 mentions)
Rhizomucor miehei lipase, by Novozymes (1 mentions)
5 protocols using rhizopus oryzae lipase
1
Lipid Profiling via TLC and Enzymatic Analysis
2
Graphene Oxide Synthesis and Functionalization
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For the preparation of graphene oxide (GO) according to the modified Hummer’s method, the chemicals including, sulfuric acid (> 98%), ortho- Phosphoric acid (85%), potassium permanganate (KMnO4), hydrogen peroxide (30%), and hydrochloric acid (37%), were purchased from Merck. Pristine graphite powder was supplied from Fluka Company. The precursors for the synthesis of the functionalized MGO were iron (II) sulfate tetrahydrate (FeSO4·4H2O, 99.7%), iron (III) chloride hexahydrate (FeCl3·6H2O, 99.0%), ammonia (NH4OH, 25 wt%), absolute ethanol, 3-aminopropyl triethoxysilane (AP), and glutaraldehyde (GA) and were purchased from Merck. Rhizopus oryzae lipase (light brown powder, ≥ 30,000 U/g) was purchased from Sigma–Aldrich. Reagents for the nano-biocatalysts assay, p-nitrophenyl palmitate (p-NPP), isopropyl alcohol (99.7%), bovine serum albumin (BSA), Coomassie Brilliant Blue G-250, Triton X-100, and Arabic gum were purchased from Merck. BG-11 medium (Blue-Green medium) components, for the cultivation and maintenance of the microalgae Chlorella vulgaris CCAP were obtained from either Merck. All organic solvents and other chemicals purchased from Merck and were of analytical grade.
3
Comparative Evaluation of Lipase Preparations
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Commercially available lipases were used as follows: (i) Immobilized lipases:
Candida antarctica lipase type B immobilized on acrylic resin (CAL-B, 7,300.0 U/g), Thermomyces lanuginosus lipase immobilized on immobead-150 (TLL, 250.0 U/g), Rhizopus oryzae lipase immobilized on immobead-150 (ROL, 340.0 U/g), Pseudomonas fluorescens lipase immobilized on immobead-150 (PFL, ≥ 600 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel (AK, ≥ 30 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel on pumice (AK, ≥ 8.0 U/g) and Amano lipase PS from Burkholderia cepacia immobilized on diatomaceous earth (PS-IM, ≥ 500 U/g) were acquired from Sigma-Aldrich®, Rhizomucor miehei lipase immobilized in anionic resin (RML, 150.0 U/g) was purchased from Novozymes ® . (ii) Unsupported lipase preparations: Pseudomonas fluorescens lipase (AK, 22,100.0 U/g), Penicillium camemberti lipase (G, 50.0 U/g), lipase from Rhizopus niveus (RNL, ≥ 1.5 U/mg), Amano lipase from Mucor javanicus (MJL, ≥ 10,000 U/g) and lipase from Aspergillus niger (ANL, 200 U/g) were acquired from Sigma-Aldrich ® . Porcine pancreas lipase (PPL, 46.0 U/g solid), and Candida rugosa lipase (CRL, 1.4 U/g) were obtained from Sigma-Aldrich ® . (iii) Esterase, immobilized in Eupergit ® C from hog liver (~200 U/g) was obtained from Sigma-Aldrich ® .
Candida antarctica lipase type B immobilized on acrylic resin (CAL-B, 7,300.0 U/g), Thermomyces lanuginosus lipase immobilized on immobead-150 (TLL, 250.0 U/g), Rhizopus oryzae lipase immobilized on immobead-150 (ROL, 340.0 U/g), Pseudomonas fluorescens lipase immobilized on immobead-150 (PFL, ≥ 600 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel (AK, ≥ 30 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel on pumice (AK, ≥ 8.0 U/g) and Amano lipase PS from Burkholderia cepacia immobilized on diatomaceous earth (PS-IM, ≥ 500 U/g) were acquired from Sigma-Aldrich®, Rhizomucor miehei lipase immobilized in anionic resin (RML, 150.0 U/g) was purchased from Novozymes ® . (ii) Unsupported lipase preparations: Pseudomonas fluorescens lipase (AK, 22,100.0 U/g), Penicillium camemberti lipase (G, 50.0 U/g), lipase from Rhizopus niveus (RNL, ≥ 1.5 U/mg), Amano lipase from Mucor javanicus (MJL, ≥ 10,000 U/g) and lipase from Aspergillus niger (ANL, 200 U/g) were acquired from Sigma-Aldrich ® . Porcine pancreas lipase (PPL, 46.0 U/g solid), and Candida rugosa lipase (CRL, 1.4 U/g) were obtained from Sigma-Aldrich ® . (iii) Esterase, immobilized in Eupergit ® C from hog liver (~200 U/g) was obtained from Sigma-Aldrich ® .
4
Enzymatic Conversion of Pangasius Fatty Oils
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Pangasius hypophthalmus oil was bought from Thien Phat Company in Tien Giang province, Vietnam. The fatty acid of the crude fish oil was neutralized by 4% sodium hydroxide solution to get the acid value of 3.75mgKOH/g oil. The iodine value was 71.4 g iodine/100g oil, DHA content was 1156mg/kg oil.
Rhizopus oryzae lipase from Sigma-Aldrich (pHopt= 7,0; Topt=37 o C, 1000U/g enzyme). Celite 545 from Sigma-Alrich was used as the support for enzyme immobilization.
Rhizopus oryzae lipase from Sigma-Aldrich (pHopt= 7,0; Topt=37 o C, 1000U/g enzyme). Celite 545 from Sigma-Alrich was used as the support for enzyme immobilization.
5
Characterization of Infant Formula Lipid Composition
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Sodium taurocholate, sodium chloride, hydrochloric acid, sodium hydroxide, potassium hydroxide, porcine pepsin, maleic acid, tris(hydroxymethyl)-aminomethane, porcine pancreatin, rhizopus oryzae lipase were purchased from Sigma-Aldrich (St Louis, MO, USA). Rabbit gastric lipase was kindly donated by Germe S.A. (Marseilles, France). The purified rHGL was donated by Bioneer A/S (Hoersholm, Denmark). The IFs used were PRENAN Stage 1 & 2 obtained from Nestlé (Lausanne, Switzerland). PRENAN stage 1 (MC-IF) is enriched with MC-TAG and PRENAN stage 2 (LC-IF) is a predominantly LC-TAG formula (Table 1). The lipid composition of the infant formulas were quantified by gas chromatography (GC) as explained below in the method section. The methylated FAs (FAME) standards used for GC were all obtained from Nu-Chek-Prep (Elysian, MN, USA). All the water used in this study was of purified Milli Q quality. All other solvents and reagents were of analytical grade or higher.
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