The ursolic acid and loganin standards were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Both the oleanolic acid and gallic acid standards were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). The HPLC-grade methanol, acetonitrile, formic acid, and water were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). The medicines and consumables for the fermentation were purchased from the Shanghai Yuanye Biotech Co., Ltd. (Shanghai, China). The B. subtilis and B. bifidum strains were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Except for the liquid chromatography reagents, all of the other experimental chemicals were of analytical grade, and double-distilled water was applied.
Oleanolic acid
Manufactured by Sangon
Sourced in China
Oleanolic acid is a pentacyclic triterpenoid compound found in various plant species. It is a naturally occurring chemical that can be isolated and used as a laboratory reagent. Oleanolic acid has chemical and physical properties that make it useful for certain research and analytical applications, but its specific intended uses should not be extrapolated or interpreted beyond a factual description of the compound.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Thermo Fisher Scientific (3 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Loganin, by Merck Group (1 mentions)
Ursolic acid, by Merck Group (1 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Gallic acid, by Sangon (1 mentions)
Rp 18 silica gel, by Merck Group (1 mentions)
Autopol 6 polarimeter, by Rudolph Research Analytical (1 mentions)
Q exactive mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Silica gel, by Qingdao Marine Chemical (1 mentions)
Versamax, by Molecular Devices (1 mentions)
2 protocols using oleanolic acid
1
Quantification of Triterpenoids and Phenolics
2
PTP1B Activity Assay Protocol
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Optical rotatory dispersion was measured on Autopol VI polarimeter (Rudolph research analytical, Hackettstown, USA). UV spectrum was obtained using a Mapada UV-1600 spectrophotometer (Shanghai, China), and IR spectrum was measured on a Nicolet iS10 FT-IR spectrophotometer (Thermo Scienetific, Massachusetts, USA) using KBr pellets. HRESIMS were performed on Q-Exactive mass spectrometer (ThermoFisher, Waltham, USA). NMR spectra were recorded on Avance III HD-500 MHz and HD-400 MHz spectrometers (Bruker, Fällanden, Switzerland) with TMS as internal standard. Column chromatography was performed over silica gel (200-300 mesh, Qingdao Marine Chemical, Qingdao, China), RP-18 silica gel (40-65 μm, Merck, Darmstadt, Germany) and Sephadex LH-20 (40-70 μm, Pharmacia, Uppsala, Sweden), respectively. Fractions or spots were observed by spraying with 10% H 2 SO 4 in EtOH followed by heating. The substrate, disodium 4-nitrophenyl phosphate, and the positive control, oleanolic acid, were purchased from Sangon Biotech (Shanghai, China), and Selleck, respectively. The PTP1B activity assay was measured on VERSAmax (Molecular Devices, USA).
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