Tritc human transferrin

The mouse anti-DENV monoclonal Ab 3H5 produced by the hybridoma HB46 (ATCC, USA), kindly supplied by Dr. Irene Bosch, University of Massachusetts Medical School, USA, and 2H2 (Chemicon, USA) were used. 3H5 is an IgG1 Ab that reacts with DENV-2 E protein and binds to IgG Fc receptor II (FcγRII) whereas 2H2 is an IgG2a Ab reactive with the prM protein of all members of DENV complex and binds to both FcγRI and FcγRII [26 (link)]. The clone AT10 of an anti-human FcγRII Ab was provided by Dr. Mirta Giordano (Academia Nacional de Medicina, Buenos Aires, Argentina).
TRITC-human transferrin was from Molecular Probes (USA) and FITC-cholera toxin B subunit was purchased from Sigma-Aldrich (USA). Chlorpromazine, dansylcadaverine, ammonium chloride, β-methylcyclodextrin, dynasore, and acridine orange were purchased from Sigma-Aldrich (USA).

The anti-DENV antibody was a mouse monoclonal antibody reactive against the E glycoprotein of the four dengue serotypes purchased from Abcam (UK). Goat anti-mouse IgG conjugated to fluorescein isothiocyanate (FITC) or rhodamine (TRITC), and TRITC-cholera toxin B subunit were purchased from Sigma-Aldrich (USA). TRITC- human transferrin was purchased from Molecular probes (USA).
Dansylcadaverine, chlorpromazine, nystatin, methyl-β-cyclodextrin, ammonium chloride, concanamycin A, dynasore, acridine orange and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (USA).