Anti cd8 yts169.4

Manufactured by BioXCell

Anti-CD8 (YTS169.4) is a monoclonal antibody that recognizes the CD8 co-receptor on T cells. It is a useful tool for the identification and isolation of CD8+ T cells.

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Lab products found in correlation

Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Anti pd 1 be0146, by BioXCell (1 mentions) Anti cd4 l3t4, by BioXCell (1 mentions) Rpmi 1640, by Gemini Bio (1 mentions) Tissue digestion buffer, by Miltenyi Biotec (1 mentions) T cell purification columns, by R&D Systems (1 mentions) Rat igg isotype control, by R&D Systems (1 mentions)

Spelling variants of this product

Anti-CD8 (28 citations) Anti-CD8 (clone YTS169.4) (18 citations) Anti-CD8 antibody (clone YTS169.4; (3 citations)

2 protocols using anti cd8 yts169.4

Murine K-RasG12Dp53null Lung Adenocarcinoma

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The murine K-RasG12Dp53null lung adenocarcinoma was isolated from lung tumors of the K-RasG12Dp53null transgenic mice obtained from J. Kurie (MD Anderson Houston, TX, USA). The culture medium (CM) was RPMI 1640 supplemented with 10% fetal bovine serum (Gemini Bioproducts West Sacramento, CA, USA), 100 units/mL penicillin, 0.1 mg/mL streptomycin, and 2 mM glutamine (JRH Biosciences Lenex, MA, USA). Fluorescein isothiocyanate-, phycoerythrin-, allophycocyanin-, PerCP-, or APC-Cy7-conjugated anti-mouse Abs to CD4 (RM4-5) and CD8a (53-6.7) were from BD Biosciences or eBiosciences. For in vivo experiments, anti-PD-1 (BE0146), anti-CD4 (L3T4), and anti-CD8 (YTS169.4) were from BioXCell. Isotype control antibody (Ab) was from Sigma. The Abs used for T cell depletion were different for monitoring T cell levels. Bradford protein quantification dye was obtained from Sigma. Tissue digestion buffer was obtained from Miltenyi and used in Miltenyi tissue homogenizer according to the manufacturer’s instructions. T cell purification columns were from R&D.

Kadam P, & Sharma S. (2020). PD-1 Immune Checkpoint Blockade Promotes Therapeutic Cancer Vaccine to Eradicate Lung Cancer. Vaccines, 8(2), 317.

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Murine Tumor Inoculation and Treatment

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5×10⁴ B16F1 (or 3×10⁵ when indicated) or 5×10⁵ CT-26 or BRAF^CAPten^−/− cells diluted in sterile PBS1X were injected subcutaneously into the shaved flank of each mouse. For metastasis experiments, 2.5×10⁴ B16F10 cells were injected intravenously in the tail vein. PTXF (Sigma) was diluted in PBS1X and sterile filtered prior to each injection. IT-603 (Calbiochem) was first diluted in EtOH for storage; prior to injections it was further diluted in Cremophor EL and then in warm PBS1X (for a final ratio of 1 EtOH: 1 Cremophor:4 PBS1X). Anti-PD-1 (RMP1-14), anti-PD-L1 (10F.9G2) and anti-CD8 (YTS.169-4) mAbs were obtained from BioXCell. Rat IgG isotype control was from R&D Systems. Mice received intraperitoneal injections of 50mg/kg PTXF, 200 μg IT-603 or 200 μg of the given mAb. In Figure 5, BALB/c mice harboring CT-26 tumors received a suboptimal dose of 100 μg of anti-PD-1.

Grinberg-Bleyer Y., Oh H., Desrichard A., Bhatt D.M., Caron R., Chan T.A., Schmid R.M., Hayden M.S., Klein U, & Ghosh S. (2017). NF-κB c-Rel Is Crucial for the Regulatory T Cell Immune Checkpoint in Cancer. Cell, 170(6), 1096-1108.e13.

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